2',7'-二氯熒光素二乙酸酯
| 中文名稱 | 2',7'-二氯熒光素二乙酸酯 |
|---|---|
| 中文同義詞 | 2',7'-二氯熒光素二乙酸酯 50MG;2,7-二氯二氫熒光素二乙酸酯;2,7-螢光素二醋酸酯;2',7'-二氯熒光素二乙酸酯;2,7-二氯二氫熒光素二乙酸;2,7-二氯二氫代熒光黃二醋酸;DCFH-DA 活性氧ROS熒光探針;2-(3,6-二乙酰氧基-2,7-二氯-9H-氧雜蒽-9-基)苯甲酸 |
| 英文名稱 | 2′,7′-Dichlorodihydrofluorescein Diacetate |
| 英文同義詞 | 2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate;2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE;2',7'-Dichlorodihydrofluoresceinediacetate;2'',7''-Dichlorofluorescein diac;2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid;2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid;2-(3,6-diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid;2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid |
| CAS號(hào) | 4091-99-0 |
| 分子式 | C24H16Cl2O7 |
| 分子量 | 487.29 |
| EINECS號(hào) | 685-843-8 |
| 相關(guān)類別 | 熒光探針，標(biāo)記，顆粒和染色;生化試劑-植物激素及核酸類;生化試劑;通用生化試劑-生物染色劑與化學(xué)指示劑;化學(xué)試劑 |
| Mol文件 | 4091-99-0.mol |
| 結(jié)構(gòu)式 |  |
2',7'-二氯熒光素二乙酸酯 性質(zhì)
2',7'-二氯熒光素二乙酸酯是一種熒光探針，作為一種非極性化合物，能夠迅速擴(kuò)散到細(xì)胞內(nèi)，細(xì)胞內(nèi)的酯酶將其水解為非熒光的二氯熒光素（DCFH），從而使探針被裝載到細(xì)胞內(nèi)。
H2DCFDA (DCFH-DA, 2',7'-Dichlorodihydrofluorescein diacetate) 是一種可滲透細(xì)胞的探針，可用于檢測(cè)細(xì)胞內(nèi) reactive oxygen species (ROS) 的產(chǎn)生。| Target | Value |
|
ROS
() |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 μM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.
安全信息
| 危險(xiǎn)品標(biāo)志 | Xi |
|---|---|
| 危險(xiǎn)類別碼 | 36/37/38 |
| 安全說明 | 22-24/25-26 |
| WGK Germany | 3 |
| F | 10-21 |
| 海關(guān)編碼 | 29329990 |