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Journal of biotechnology

Journal of biotechnology

IF: 4.09
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Efficient biosynthesis of (R)-3-amino-1-butanol by a novel (R)-selective transaminase from Actinobacteria sp.

Published:10 April 2019 DOI: 10.1016/j.jbiotec.2019.02.008 PMID: 30853639
Xiao-Ling Tang , Nan-Nan Zhang , Guo-Yan Ye , Yu-Guo Zheng

Abstract

(R)-3-amino-1-butanol is a key intermediate of Dolutegravir for the treatment of HIV/AIDS and its green and efficient biosynthesis has attracted a great deal of attention. Transaminases are currently used as promising biocatalyst for the synthesis of chiral amines. However, many transaminases have (S)-specificity and (R)-selective transaminases were less exploited and studied, making the production of (R)-amines remain challenging. In this study, a novel transaminase from Actinobacteria sp. (As-TA) was obtained and applied for the biosynthesis of (R)-3-amino-1-butanol by transferring the amino group from isopropylamine to 4-hydroxy-2-butanone. After optimization of the reaction condition and using a substrate fed-batch strategy, the conversion of 100, 200, 300, 400 and 500?mM 4-hydroxy-2-butanone reached 100%, 94.9%, 86.1%, 76.1% and 70.9%, respectively. (R)-3-amino-1-butanol with a maximum yield of 29.6?g/L and 99.9% e.e. value was obtained. This was the first time demonstrating the successful biosynthesis of (R)-3-amino-1-butanol with transaminase as biocatalyst and the obtained As-TA enriched the enzyme pool of transaminase with (R)-specificity.

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Related products
Procduct Name CAS Molecular Formula Supplier Price
Isopropylamine 75-31-0 C3H9N 461 suppliers $14.00-$675.00
(R)-3-amino-1-butanol 61477-40-5 C4H11NO 452 suppliers $15.00-$1190.00
4-Hydroxy-2-butanone 590-90-9 C4H8O2 344 suppliers $10.00-$859.90
Granuliberin R peptide 6 suppliers Inquiry

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