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Journal of biotechnology

Journal of biotechnology

IF: 4.09
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Degradation of nonphenolic lignin by the laccase/1-hydroxybenzotriazole system

Published:25 August 2000 DOI: 10.1016/S0168-1656(00)00303-5 PMID: 10989177
Ewald Srebotnik , Kenneth E Hammel

Abstract

Phenolic and nonphenolic (permethylated) synthetic [14C]lignins were depolymerized by Trametes villosa laccase in the presence of a radical mediator, 1-hydroxybenzotriazole (HOBT). Gel permeation chromatography of the treated lignins showed that ~10% of their substructures were cleaved. The system also cleaved a β-O-4-linked model compound, 1-(4-ethoxy-3-methoxy-ring-[14C]phenyl)-2-(2-methoxyphenoxy)-propane-1,3-diol, and a β-1-linked model, 1,2-bis-(3-methoxy-4-[14C]methoxyphenyl)-propane-1,3-diol, that represent nonphenolic substructures in lignin. High performance liquid chromatography of products from the oxidized models showed that they were produced in sufficient yields to account for the ability of laccase/HOBT to depolymerize nonphenolic lignin.

Substances (3)

Related products
Procduct Name CAS Molecular Formula Supplier Price
1-Hydroxybenzotriazole 2592-95-2 C6H5N3O 787 suppliers $14.14-$693.00
Laccase 80498-15-3 C9H13NO 191 suppliers $106.00-$920.00
2-(2-methoxyphenoxy)propane-1,3-diol 14007-09-1 C10H14O4 73 suppliers $323.00-$2200.00

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