午夜插插,噜噜噜影院,啪啪伊人网,欧美熟夫,景甜吻戏视频,男人强操性感蕾丝美女视频在线网站,日本美女跳舞视频

ChemicalBook >> journal list >> Journal of pharmaceutical and biomedical analysis >>article
Journal of pharmaceutical and biomedical analysis

Journal of pharmaceutical and biomedical analysis

IF: 3.1
Download PDF

Dynamic profiling of intra-and extra-cellular L/D-amino acids metabolism in colorectal cell and intestinal epithelial cell

Published:9 December 2024 DOI: 10.1016/j.jpba.2024.116622 PMID: 39657277
Wenchan Deng,?Rongrong Huang,?Yuanjiang Pan,?Cuirong Sun

Abstract

The metabolism process of amino acids is closely related to the growth of normal and cancer cells. It is still not clear how L/D-configuration amino acids participate in the metabolism of colorectal cell. Herein, intra- and extra-cellular metabolic distribution of L/D-amino acids in colorectal cell (HCT116) and human normal intestinal epithelial cell (NCM460) were profiled utilizing HPLC-MS/MS coupled with a chiral probe. The results displayed the differential metabolic portrayal for the two cell lines. Compared with NCM460 cell, 13 kinds of significant differential amino acids were founded in a lower concentration within HCT116 cell, and L-Gln was even not detected for intra-cell; as for extra-cell culture medium, the HCT116 cell consumed more L-Gln, D-Phe and D-Leu, while L-Met was low ingested in HCT116 cell. L-Ala and Gly were excretion in both two cell lines, excepted L-Cit which was uptake in HCT116 and excretion in NCM460 cell. Furthermore, the dynamic changes of chiral amino acids displayed that phenylalanine, tyrosine and tryptophan biosynthesis and arginine biosynthesis is the major pathway for intra-cellular metabolites and extra-cellular metabolites, respectively. Moreover, with additional D-amino acids in culture medium, the results exhibited that high concentration of D-amino acids have no significant effect on the proliferation of NCM460 cell, but could influence the profiling of amino acids metabolites, and further affect the proliferation of HCT116 cell. This present work enhances the understanding of these differential amino acids metabolic network and depicts a dynamic process of metabolic dysregulation of HCT116 and NCM460 cell.

Substances (6)

Materials
Procduct Name CAS Molecular Formula Supplier Price
L-Isoleucine 73-32-5 C6H13NO2 1058 suppliers $5.00-$20140.00
L-Methionine 63-68-3 C5H11NO2S 1020 suppliers $5.00-$11583.00
L-Valine 72-18-4 C5H11NO2 982 suppliers $5.00-$15223.00
L-Alanine 56-41-7 C3H7NO2 949 suppliers $5.00-$4168.00
DL-Serine 302-84-1 C3H7NO3 640 suppliers $5.00-$1456.00
FETUIN 9014-81-7 NULL 66 suppliers $37.07-$4380.00

Similar articles

IF:5.3

Pevonedistat (MLN4924): mechanism of cell death induction and therapeutic potential in colorectal cancer.

ACS Applied Nano Materials Jennifer Ferris, Margarita Espona-Fiedler,etc Published: 21 July 2020
IF:3.5

Engineering substrate and energy metabolism for living cell production of cytidine-5′-diphosphocholine

Biotechnology and Bioengineering Yanna Ren, Qi Liu,etc Published: 30 January 2020