溴化乙啶
| 中文名稱 | 溴化乙啶 |
|---|---|
| 中文同義詞 | 溴化乙腚;溴化-3,8-二氨基-5-乙基-6-苯基菲啶翁;溴化乙啶;溴化乙啶/溴乙菲啶;溴化乙啶(EB);溴化乙錠, 98%(DRY WT.);胡米溴銨;EB,溴化乙啶 |
| 英文名稱 | Ethidium bromide |
| 英文同義詞 | 3,8-diaMino-5-ethyl-6-phenylphenanthridin-5-iuM broMide;EthidiuM broMide dropper bottle,5Ml;2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide, 1% aqueous solution;Ethidinium bromide, 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide;EthidiuM BroMide, OMniPur(R);EthidiuM broMide, pure, 95% 1GR;EthidiuM broMide, pure, 95% 5GR;Dromilac |
| CAS號(hào) | 1239-45-8 |
| 分子式 | C21H20BrN3 |
| 分子量 | 394.32 |
| EINECS號(hào) | 214-984-6 |
| 相關(guān)類別 | 細(xì)胞生物;用于生產(chǎn)的化學(xué)試劑;基因組學(xué)和分子診斷;微生物;小分子抑制劑;生物化學(xué);Amines;Aromatics;Intermediates & Fine Chemicals;Pharmaceuticals;生化試劑;色素類;marker;其他生化試劑;熒光探針，標(biāo)記，顆粒和染色;無(wú)機(jī)化合物和鹽;Phenanthridine;分子生物學(xué);Purines |
| Mol文件 | 1239-45-8.mol |
| 結(jié)構(gòu)式 |  |
溴化乙啶 性質(zhì)
| 熔點(diǎn) | 260-262 °C (dec.)(lit.) |
|---|---|
| 密度 | 1.3739 (rough estimate) |
| 堆積密度 | 340kg/m3 |
| 折射率 | 1.6700 (estimate) |
| 閃點(diǎn) | >100°C |
| 儲(chǔ)存條件 | 2-8°C |
| 溶解度 | H2O:10 mg/mL,不透明,深紅色 |
| 形態(tài) | 粉末 |
| 顏色 | 紅色至深紫色 |
| 氣味 (Odor) | 無(wú)味固體 |
| PH值 | 4.4 (20°C, 10g/L in H2O) |
| 水溶解性 | 40 g/L (25 ºC) |
| 最大波長(zhǎng)(λmax) | 518 nm, 210 nm, 285 nm, 316 nm, 343 nm, 480 nm, 525 nm |
| Merck | 14,4731 |
| BRN | 3642536 |
| 穩(wěn)定性 | 穩(wěn)定的。與強(qiáng)氧化劑不相容。 |
| 生物領(lǐng)域應(yīng)用 | Nucleic acid hybridization; detecting nucleic acids,cells,cancer cells,human cytomegalovirus,hydrogenase A (hydA) of Clostridia,influenza A virus,oligonucleotides,viable Plesiomonas shigelloides; apoptosis assay; nucleic acid quantification |
| 主要應(yīng)用 | Electroluminescent displays;photoresists |
| CAS 數(shù)據(jù)庫(kù) | 1239-45-8(CAS DataBase Reference) |
| EPA化學(xué)物質(zhì)信息 | Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1239-45-8) |
熒光分析法可分為直接測(cè)定法和間接測(cè)定法二種。直接測(cè)定法是利用物質(zhì)自身發(fā)射的熒光，即所謂“自熒光”或“內(nèi)源熒光”來(lái)進(jìn)行測(cè)定。但在自然界中只有少部分物質(zhì)在光激發(fā)后可以發(fā)射熒光，而有相當(dāng)一部分物質(zhì)發(fā)熒光很弱或不發(fā)射熒光，如蛋白和核酸就是發(fā)熒光很弱的物質(zhì)。為了充分利用熒光分析法靈敏度高這一特點(diǎn)就需使其轉(zhuǎn)化成熒光物質(zhì)再進(jìn)行測(cè)定。此時(shí)可用某些試劑(如熒光染料) 使其與熒光較弱或不顯熒光的物質(zhì)共價(jià)或非共價(jià)結(jié)合，以形成發(fā)熒光的結(jié)合物再進(jìn)行測(cè)定，這就是所謂的“熒光探針”技術(shù)或“熒光標(biāo)記”技術(shù)。使試劑(熒光劑) 吸附在大分子上或共價(jià)結(jié)合到大分子上。所用的染料溴化乙啶被叫做“熒光探劑”。探劑熒光特性的變化，實(shí)際上是由于大分子構(gòu)象的變化所引起的探劑環(huán)境變化的信號(hào)，所以利用這種信號(hào)還可以得到大分子構(gòu)象變化的信息。Ethidium Bromide (Homidium bromide, EtBr, EB) 是一種嵌入劑，與DNA堿基對(duì)類似。EtBr在分子生物學(xué)實(shí)驗(yàn)技術(shù)（如瓊脂糖凝膠電泳）中經(jīng)常被用于熒光標(biāo)記（核酸染色）。
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The preparation of agarose gel:
1. Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated.
2. Mix the agarose powder with 1X TAE/TBE.
3. Microwave for 1-3 min until the agarose is completely dissolved (
Caution
: not overboil).
4. Make the solution cool down before solidification.
5. Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 μL stock/100 mL gel).
6. Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light.
CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration. Ethidium bromide has been used in a number of fluorimetric assays for nuc leic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprot ozoal (Trypanosoma).
安全信息
| 危險(xiǎn)品標(biāo)志 | T,T+ |
|---|---|
| 危險(xiǎn)類別碼 | 23-68-36/37/38-26-21/22-22 |
| 安全說(shuō)明 | 36/37-45-36/37/39-28A-26-22-28-63 |
| 危險(xiǎn)品運(yùn)輸編號(hào) | UN 2811 6.1/PG 1 |
| WGK Germany | 3 |
| RTECS號(hào) | SF7950000 |
| F | 8-9 |
| 危險(xiǎn)等級(jí) | 6.1 |
| 包裝類別 | I |
| 海關(guān)編碼 | 29339990 |
| 毒害物質(zhì)數(shù)據(jù) | 1239-45-8(Hazardous Substances Data) |
| 毒性 | Intercalating dye widely used to stain DNA in gels and gradients. The DNA can be visualized readily by irradiation with ultraviolet light, as little as 0.5 μg of DNA being detectable by such methods. Ethidium bromide is also added to cesium chloride density gradients, since as an intercalating molecule it binds more readily to linear DNA than to closed collinear circles of DNA (such as plasmids). Binding of ethidium bromide reduces the density of DNA, thus covalent circles of DNA have higher densities at saturating concentrations of ethidium bromide, permitting the separation of plasmid DNA. Ethidium bromide is a putative carcinogen. |
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