VEGFC Rabbit Polyclonal Antibody
Rabbit
Human, Mouse, Rat
Recombinant protein encompassing a sequence within the center region of human VEGFC.
WB, IHC-P
VEGFC antibody---GTX113574 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human hepatoma, using VEGFC(GTX113574) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D.
Anti-ACTA2 Rabbit Monoclonal Antibody是一類可以特異性結(jié)合Anti-ACTA2的單克隆抗體,主要用于檢測Anti-ACTA2的Western Blot、IHC-P、IF、ELISA、Co-IP等多種免疫學(xué)實(shí)驗(yàn)。
雙抗體夾心法測定標(biāo)本中Anti-ACTA2水平。用純化的Anti-ACTA2抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入Anti-ACTA2,再與HRP標(biāo)記的Anti-ACTA2抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的Anti-ACTA2呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計(jì)算樣品中Anti-ACTA2濃度。