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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Oseltamivir  Impurity 67 Oseltamivir Impurity 67
Ixazomib Impurity ABCDEFGHJKL Ixazomib Impurity ABCDEFGHJKL
2-[[[4-(2,2-dimethyl-1-oxopropoxy)phenyl]sulfonyl]amino]-Benzoic acid 2-[[[4-(2,2-dimethyl-1-oxopropoxy)phenyl]sulfonyl]amino]-Benzoic acid 188114-98-9 C18H19NO6S
Dapagliflozin Dapagliflozin 639852-30-5 C19H44O5Si4
Cabozantinib impurity 52 Cabozantinib impurity 52
Canagliflozin Impurity 71 Canagliflozin Impurity 71
N-((4-(5-methyl-2-phenyloxazol-4-yl)phenyl)sulfonyl)propionamide N-((4-(5-methyl-2-phenyloxazol-4-yl)phenyl)sulfonyl)propionamide 2242749-02-4 C19H18N2O4S
Dapoxetine impurity 67 Dapoxetine impurity 67
2-(5-Acetyl-2-ethoxyphenyl)-5-methyl-7-propyl-imidazo[5,1-f][1,2,4]triazin-4(1H)-one 2-(5-Acetyl-2-ethoxyphenyl)-5-methyl-7-propyl-imidazo[5,1-f][1,2,4]triazin-4(1H)-one 358390-43-9 C19H22N4O3
Olopatadine Ethyl Ester Olopatadine Ethyl Ester 113806-03-4 C23H27NO3
Empagliflozin Impurity 80 Empagliflozin Impurity 80
Empagliflozin Impurity 92 Empagliflozin Impurity 92
Iohexol Impurity N Iohexol Impurity N
Pitavastatin 5-Oxo Impurity  Calcium Salt Pitavastatin 5-Oxo Impurity Calcium Salt 2180095-22-9 C25H24CaFNO4
1-(2-bromo-3,5-dihydroxyphenyl)ethanone 1-(2-bromo-3,5-dihydroxyphenyl)ethanone 2092614-65-6 C8H7BrO3
Lenvatinib impurity LFZZ-18 Lenvatinib impurity LFZZ-18 C11H8Cl2N2O2
Rosuvastatin Impurity 121 Rosuvastatin Impurity 121 140164-51-8 C10H18O5
Brivaracetam Impurity 38 Brivaracetam Impurity 38
6-HYDROXY ONDANSETRON 6-HYDROXY ONDANSETRON 110708-17-3 C18H19N3O2
2-(3-cyano-4-isobutoxyphenyl)-4-methylthiazole-5-carboxylic acid 1-oxide 2-(3-cyano-4-isobutoxyphenyl)-4-methylthiazole-5-carboxylic acid 1-oxide 2418591-42-9 C16H16N2O4S
Ropivacaine impurity Ropivacaine impurity 1786841-18-6 C12H14N2O3
Isavuconazole Impurities 04 Isavuconazole Impurities 04
Ticagrelor Impurity 119 Ticagrelor Impurity 119
Ticagrelor Impurity 118 Ticagrelor Impurity 118
D-erythro-Hexonic acid, 2,4-dideoxy-3,5-O-(1-methylethylidene)-, acetate (9CI) D-erythro-Hexonic acid, 2,4-dideoxy-3,5-O-(1-methylethylidene)-, acetate (9CI) 402508-35-4 C11H18O6
L-Alanine, 3-[(S)-(carboxymethyl)sulfinyl]- L-Alanine, 3-[(S)-(carboxymethyl)sulfinyl]- 112246-66-9 C5H9NO5S
2-Propanone, 1-[4,5-dihydro-2-(4-methyl-1-piperazinyl)-4-thioxo-3H-1,5-benzodiazepin-3-ylidene]- 2-Propanone, 1-[4,5-dihydro-2-(4-methyl-1-piperazinyl)-4-thioxo-3H-1,5-benzodiazepin-3-ylidene]- 1902955-67-2 C17H20N4OS
3(2H)-Pyridazinone, 6-[4-(ethylamino)phenyl]-4,5-dihydro-5-methyl- 3(2H)-Pyridazinone, 6-[4-(ethylamino)phenyl]-4,5-dihydro-5-methyl- 52240-42-3 C13H17N3O
Benzoic acid, 4-[[4-(aminocarbonyl)-1-piperidinyl]methyl]- Benzoic acid, 4-[[4-(aminocarbonyl)-1-piperidinyl]methyl]- 938143-02-3 C14H18N2O3
1-(4-methylbenzyl)-1,4,8,11-tetraazacyclotetradecane tetrahydrochloride 1-(4-methylbenzyl)-1,4,8,11-tetraazacyclotetradecane tetrahydrochloride
2,4-bis(1-(2,3-dimethylphenyl)ethyl)-1H-imidazole 2,4-bis(1-(2,3-dimethylphenyl)ethyl)-1H-imidazole
2-((4R,6S)-6-((2-((4R,6S)-6-(hydroxymethyl)-2,2-dimethyl-1,3-dioxan-4-yl)acetoxy)methyl)-2,2-dimethyl-1,3-dioxan-4-yl)acetic acid 2-((4R,6S)-6-((2-((4R,6S)-6-(hydroxymethyl)-2,2-dimethyl-1,3-dioxan-4-yl)acetoxy)methyl)-2,2-dimethyl-1,3-dioxan-4-yl)acetic acid
1,3-bis(4-((E)-3-methoxy-3-oxoprop-1-en-1-yl)benzyl)-1H-imidazol-3-ium bromide 1,3-bis(4-((E)-3-methoxy-3-oxoprop-1-en-1-yl)benzyl)-1H-imidazol-3-ium bromide
Crisaborole intermediate Crisaborole intermediate 1187230-70-1 C14H14BNO3
Glycine, (2R)-N-[(4-ethyl-2,3-dioxo-1-piperazinyl)carbonyl]-2-phenylglycyl-2-[(2S,4S)-4-carboxy-5,5-dimethyl-2-thiazolidinyl]-, (2R)- Glycine, (2R)-N-[(4-ethyl-2,3-dioxo-1-piperazinyl)carbonyl]-2-phenylglycyl-2-[(2S,4S)-4-carboxy-5,5-dimethyl-2-thiazolidinyl]-, (2R)- 1449784-97-7 C23H29N5O8S
6-Quinolinecarboxamide, 4-[(2-chloro-4-hydroxyphenyl)amino]-7-methoxy- 6-Quinolinecarboxamide, 4-[(2-chloro-4-hydroxyphenyl)amino]-7-methoxy- 2380197-89-5 C17H14ClN3O3
1-Pyrrolidineacetic acid, α-ethyl-2-oxo-4-propyl-, methyl ester, (4R)- 1-Pyrrolidineacetic acid, α-ethyl-2-oxo-4-propyl-, methyl ester, (4R)- 930123-52-7 C12H21NO3
Traxipide impurity Traxipide impurity
Benzeneacetic acid, 4-[2-[4-[1-(2-ethoxyethyl)-1H-benzimidazol-2-yl]-1-piperidinyl]ethyl]-α,α-dimethyl-, methyl ester Benzeneacetic acid, 4-[2-[4-[1-(2-ethoxyethyl)-1H-benzimidazol-2-yl]-1-piperidinyl]ethyl]-α,α-dimethyl-, methyl ester 1181267-38-8 C29H39N3O3
Benzenemethanol, 4-[2-(1-methylethoxy)ethoxy]- Benzenemethanol, 4-[2-(1-methylethoxy)ethoxy]- 1039828-27-7 C12H18O3
Brivaracetam Impurity 7 HCl Brivaracetam Impurity 7 HCl 357338-46-6 C13H27ClN2O3
Bisacodyl Impurity 1 Bisacodyl Impurity 1 13004-51-8 C18H15NO2
Vecuronium Bromide ImpurityACDEFGHJKL Vecuronium Bromide ImpurityACDEFGHJKL
Butanedioic acid, 2-[[2-methyl-1-(phenylmethyl)-1H-imidazol-5-yl]methylene]-, 1-ethyl ester, (2E)- Butanedioic acid, 2-[[2-methyl-1-(phenylmethyl)-1H-imidazol-5-yl]methylene]-, 1-ethyl ester, (2E)- 1640981-02-7 C18H20N2O4
INDEX NAME NOT YET ASSIGNED INDEX NAME NOT YET ASSIGNED 2452300-94-4 C21H25ClO8
1-Butanone, 1-(4-fluorophenyl)-4-[(6bS,10aS)-2,3,6b,9,10,10a-hexahydro-3-methyl-1H-pyrido[3',4':4,5]pyrrolo[1,2,3-de]quinoxalin-8(7H)-yl]- 1-Butanone, 1-(4-fluorophenyl)-4-[(6bS,10aS)-2,3,6b,9,10,10a-hexahydro-3-methyl-1H-pyrido[3',4':4,5]pyrrolo[1,2,3-de]quinoxalin-8(7H)-yl]- 1576240-15-7 C24H28FN3O
1-Pyrrolidineacetamide, α-ethyl-4-(1-methylethyl)-2-oxo-, (αS,4S)- 1-Pyrrolidineacetamide, α-ethyl-4-(1-methylethyl)-2-oxo-, (αS,4S)- 357334-83-9 C11H20N2O2
impurity 42 impurity 42
2,2'-(4,10-bis((2S,3R)-1,3,4-trihydroxybutan-2-yl)-1,4,7,10-tetraazacyclododecane-1,7-diyl)diacetic acid 2,2'-(4,10-bis((2S,3R)-1,3,4-trihydroxybutan-2-yl)-1,4,7,10-tetraazacyclododecane-1,7-diyl)diacetic acid
Loratadine Impurity 2 Loratadine Impurity 2 167891-69-2 C22H25ClN2O2
Eliglustat (1S,2S)-Isomer (Hemitartrate) Eliglustat (1S,2S)-Isomer (Hemitartrate) 1092472-70-2 C23H36N2O4
3-Azetidineacetic acid, 1-(ethylsulfonyl)-3-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl]- 3-Azetidineacetic acid, 1-(ethylsulfonyl)-3-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl]- 2271228-52-3 C16H18N6O4S
Cyclopentanecarboxylic acid, 3-[(1S)-1-amino-2-ethylbutyl]-4-[(aminoiminomethyl)amino]-2-hydroxy-, (1S,2S,3S,4R)- Cyclopentanecarboxylic acid, 3-[(1S)-1-amino-2-ethylbutyl]-4-[(aminoiminomethyl)amino]-2-hydroxy-, (1S,2S,3S,4R)- 2412376-06-6 C13H26N4O3
Peramivir Impurity 49 Peramivir Impurity 49 2758048-81-4 C14H26N2O4
8-Chloro-5,6-dihydro-11H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-one 8-Chloro-5,6-dihydro-11H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-one 31251-41-9 C14H10ClNO
(R)-Ornidazole (R)-Ornidazole 166734-80-1 C7H10ClN3O3
Apixaban Impurity 22 Apixaban Impurity 22 1449510-64-8 C25H26ClN5O4
Cefdinir impurity TU mixture Cefdinir impurity TU mixture
Fotagliptin Impurity 1 Fotagliptin Impurity 1
Rocuronium bromide Impurity 7 Rocuronium bromide Impurity 7
Tofacitinib Impurity 69 Tofacitinib Impurity 69
Benzenamine, 3-(2-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)- Benzenamine, 3-(2-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)- 641571-06-4 C11H10F3N3
Ciprofloxacin EP Impurity E Ciprofloxacin EP Impurity E 879005-96-6 C16H20FN3O
ethyl 2-(3-cyano-4-propoxyphenyl)-4-methylthiazole-5-
carboxylate ethyl 2-(3-cyano-4-propoxyphenyl)-4-methylthiazole-5- carboxylate C17H18N2O3S
(2S,5R,6S)-6-bromo-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Diphenylmethyl Ester (2S,5R,6S)-6-bromo-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid Diphenylmethyl Ester 74189-25-6 C21H20BrNO3S
Trelagliptin Impurity 5 Trelagliptin Impurity 5 2087874-92-6 C22H24FN5O5
9H-Purin-6-amine, 9-beta-D-arabinofuranosyl-2-methoxy- 9H-Purin-6-amine, 9-beta-D-arabinofuranosyl-2-methoxy- 51742-84-8 C11H15N5O5
(E)-4-BROMO-N,N-DIMETHYLBUT-2-ENAMIDE (E)-4-BROMO-N,N-DIMETHYLBUT-2-ENAMIDE 137131-09-0 C6H10BrNO
(E)-4-BROMO-N,N-DIMETHYLBUT-2-ENAMIDE (E)-4-BROMO-N,N-DIMETHYLBUT-2-ENAMIDE 137131-09-0 C6H10BrNO
Almotriptan Impurity 1 Almotriptan Impurity 1 334981-13-4 C17H25N3O3S
Almotriptan Impurity 1 Almotriptan Impurity 1 334981-13-4 C17H25N3O3S
Amlodipine Impurity 33 Amlodipine Impurity 33 C26H31ClN2O8S
Erlotinib Impurity 36 HCl Erlotinib Impurity 36 HCl
Rasagiline Impurity 6 HCl Rasagiline Impurity 6 HCl 138911-07-6 C9H11NO
Trelagliptin Impurity SJ Trelagliptin Impurity SJ 2087874-94-8 C40H42F2N10O6
Eliglustat Impurity 2(Eliglustat SR-Isomer) Eliglustat Impurity 2(Eliglustat SR-Isomer) 1092472-66-6 C23H36N2O4
Tamsulosin impurity C Tamsulosin impurity C C20H28N2O5S
Umeclidinium Bromide Impurity 9 DiHCl Umeclidinium Bromide Impurity 9 DiHCl 494778-36-8 C18H32N2O4
Flucloxacillin Impurity 4 Flucloxacillin Impurity 4
Ipratropium bromide Impurity 4 Ipratropium bromide Impurity 4 C20H29Br2NO3
Cefminox Impurity 6 Cefminox Impurity 6
Afatinib Impurity 55 Afatinib Impurity 55
Apremilast Impurity 30 Apremilast Impurity 30 1609546-91-9
Aprepitant Impurity 33 Aprepitant Impurity 33 502537-39-5 C20H20F7NO3
Brivaracetam Impurity 9 Brivaracetam Impurity 9
Esomeprazole Impurity 43 Esomeprazole Impurity 43
Etoricoxib Impurity 40 Etoricoxib Impurity 40
Lenvatinib Impurity 31 Lenvatinib Impurity 31
Oseltamivir Impurity 35 Oseltamivir Impurity 35 757965-01-8 C14H22O4
Palbociclib Impurity 39 Palbociclib Impurity 39
Posaconazole Impurity 75 Posaconazole Impurity 75
Rabeprazole Impurity 22 Rabeprazole Impurity 22
Sunitinib Impurity 12 Sunitinib Impurity 12
Ticagrelor Impurity 113 Ticagrelor Impurity 113
Ticagrelor Impurity 97 Ticagrelor Impurity 97
Tofacitinib Impurity 117 Tofacitinib Impurity 117
Trelagliptin Impurity 22 Trelagliptin Impurity 22
Trelagliptin Impurity 29 Trelagliptin Impurity 29
Vildagliptin Impurity 43 Vildagliptin Impurity 43
Dexchlorpheniramine impurity F Dexchlorpheniramine impurity F
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