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????? ??? ???
?? ??:
553-24-2
???:
?????
???(??):
C.I.?????5;C.I.?????5,???????;N8,N8,3-????-2,8-?????????????;??????;2,8-??????,N8,N8,3-????-,???????;?????;??????-??????????????
???:
Neutral Red
???(??):
Neutral Red;N2,N2,7-TriMethylphenazine-2,8-diaMine hydrochloride;Kernechrot;C.I. 50040;neutralredw;Basic Red 5;c.i.basicred5;michromeno.226;cervenneutralni;cervenzasadita5
CBNumber:
CB7853810
???:
C15H17ClN4
??? ??:
288.78
MOL ??:
553-24-2.mol
MSDS ??:
SDS

????? ??

???
290 °C (dec.) (lit.)
?? ?
441.31°C (rough estimate)
??
1.1590 (rough estimate)
?? ??
350-500kg/m3
???
1.6110 (estimate)
?? ??
Store at +5°C to +30°C.
???
H2O: ???10mg/mL
??? ??
??
??? ??
??? ??
?? ?? ??
50040
?? ?? (pKa)
6.7, 7.4(at 25℃)
??
?? ?? ??
pH ??
6.8(red)-8(yellow)
??????(pH)
3.1 (10g/l, H2O, 20℃)
??
?? ??
???
50g/L
?? ??(λmax)
540nm, 533nm, 544nm, 529nm, 454nm
Merck
14,6488
BRN
3918740
???
????. ?? ???? ???? ????.
??? ??? ??
Detecting pathogens,bacterial infections; treating age-related macular degeneration,burns,cancer,diabetes,obesity,fungal infections,viral diseases
?? ??
Fuel cell power generation system, liquid crystal displays, solor cells, sensors, thermochromic materials, coloring wood, detergents, assessment of tobacco smoke, cosmetics, detect bacterial infections, multidrug resistance inhibitors, treatment of burns, endodontic, diabetes, obesity, cancer, age-related macular degeneration, viral diseases
CAS ??????
553-24-2(CAS DataBase Reference)
EPA
Neutral Red (553-24-2)
??
  • ?? ? ?? ??
  • ?? ? ???? ?? (GHS)
??? ?? Xn,Xi
?? ???? ?? 68-36/37/38
????? 36/37-37/39-36-26-24/25
WGK ?? 3
RTECS ?? SG1400000
TSCA Yes
HS ?? 32041300
?? LD50 intraperitoneal in mouse: 432mg/kg
????(GHS): GHS hazard pictograms
?? ?: Warning
??·?? ??:
?? ??·?? ?? ?? ?? ?? ?? ? ?? ?? P- ??
H315 ??? ??? ??? ????? ?? ????? ?? 2 ?? GHS hazard pictograms P264, P280, P302+P352, P321,P332+P313, P362
H319 ?? ?? ??? ??? ?? ? ?? ?? ??? ?? ?? 2A ?? GHS hazard pictograms P264, P280, P305+P351+P338,P337+P313P
H335 ?? ???? ??? ? ?? ?? ???? ?? - 1? ??;???? ?? ?? 3 ?? GHS hazard pictograms
??????:
P280 ????/???/???/?????? ?????.
P302+P352 ??? ??? ??? ?? ????.

????? MSDS


Neutral Red

????? C??? ??, ??, ??

??? ??

dark green powder

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Neutral Red is a common histology stain used to determine cell viability. It is also used as indicator for nitrogen determination; pH indicator; Dyes and metabolites.

?? ??

Transition of pH Indicator: pH 6.8 - 8.0, red to yellow. Basic red 5 is a dye used in histology for staining nuclei. It is also a pH indicator. As such it is used in microbial culture media for differentation of bacteria. For example MacConkey agar contains it and lactose. Acidification by lactic acid turns on the Basic red 5. Therefore, colonies formed by bacteria that ferment lactose into lactic acid will turn red and stand out from the pale colonies of non-lactose-fermenting bacteria. Basic red 5 is also used in cell viability tests based on the ability to incorporate and bind it.

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ChEBI: Neutral red is a hydrochloride obtained by combining the free base of neutral red with one equivalent of hydrochloric acid. Neutral red acts as a pH indicator, changing from red to yellow between pH 6.8 and 8.0. It has a role as a dye, an acid-base indicator and a two-colour indicator. It contains a neutral red(1+).

?? ??

(a) N,N-dimethyl-4-nitrosobenzenamine?and 4-Methylbenzene-1,3-diamine???reaction, form Toluylene Blue (Basic Dye, C.I. 49410), heating make cyclization; (b) oxidation N1,N1-dimethylbenzene-1,4-diamine?, and 4-Methylbenzene-1,3-diamine mixture.

?? ??

Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

Staining Procedures

Neutral Red is a non-toxic stain used for studies of living cells, for example to observe plasmolysis. The stain is used as an aqueous solution (0.1 %, w/v) and stains lipids in vacuoles and certain cell walls.

Purification Methods

In aqueous solution it is red at pH 6.8 and yellow at pH 8.0. [Beilstein 25 III/IV 3054.]

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