Alkaline Phosphatase (AP), activated Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
The reagent can be used for labeling water-soluble substances with reactive and accessible primary amino groups (
e.g., peptides or proteins) with alkaline phosphatase for use in analytical methods.
It is particularly suitable for the coupling of antibodies with alkaline phosphatase, as the resulting conjugate is optimal for use in immunochemical detection systems, for example, ?ELISA, immunohistochemistry, and immunoblotting procedures.
The enzyme can be used without pre-activation for the labeling of Ig, Ig Fab, and Ig F(ab′)
2 fragments from rabbit, mouse, sheep, and goat.
Allgemeine Beschreibung
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
The quantity of enzyme is sufficient to conjugate approx. 2 mg immunoglobulin G (IgG). Roche recommends dividing the total quantity to be conjugated into 5 portions; each portion provides 0.3 to 0.5 ml of conjugate from approx. 0.35 mg IgG; this quantity can be diluted 1:4,000 to 1:10,000 for ELISA applications.
ContentsAlkaline phosphatase from calf intestine lyophilizate from 0.5 ml.
Alkaline Phosphatase (AP), activated Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
