Genopure PlasMid Midi Kit Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
The Genopure Plasmid Midi Kit prepares transfection-grade plasmid DNA in medium quantities (up to 100 μg plasmid) from bacterial cultures. Isolated plasmid is suitable for most molecular biology applications:
- Transfection
- Southern blotting
- Sequencing
- PCR
- Restriction analysis
- Cloning
Allgemeine Beschreibung
Plasmid recovery was tested with 50 μg purified plasmid. The recovery was >90%, with more than 80% in supercoiled form. The yield of plasmid DNA was determined by isolating pBS from DH5a cells. From 30 mL culture volume with a density of A600 between 3 and 6, >85 μg of plasmid DNA was obtained.
The purity (checked by the ratio of A260/A280) is 1.8 + 0.2.
No RNA is detectable.
The kit components have been tested for the absence of nucleases according to current quality control procedures.
Genopure PlasMid Midi Kit Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
