KAPA Taq HotStart Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

KAPA Taq HotStart^? may be used in:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• Polymerase chain reaction (PCR)

Allgemeine Beschreibung

KAPA Taq is supplied in a 2X ReadyMix^? format containing all the components required for PCR except primers and template-simply use PCR-grade water to make up the required reaction volume. KAPA Taq ReadyMix is also available with loading dye reaction buffer, allowing, you to load your PCR product directly onto the agarose gel with no extra steps for adding loading/tracking dye. KAPA Taq DNA polymerase is the single-subunit Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus, purified from recombinant Escherichia coli.

Biochem/physiol Actions

KAPA Taq and KAPA Taq HotStart? DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. KAPA Taq hotStart buffer is a uniquely formulated buffer to facilitate specific primer annealing. This translates to higher yields of a specific product when compared to traditional Taq buffers, and improved amplification of GC- and AT-rich templates. However, KAPA Taq hotStart DNA polymerase may be used in combination with any standard Taq buffer with a pH of 8.3 or higher.

KAPA Taq HotStart Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

KAPA Taq HotStart Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51456	80
Merck KGaA	(+86) 21 2033 8288	sigmaname@qq.com	CHINA	292394	58

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• KAPA Taq HotStart