A549 Cells GFP-SMAD4 Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
This product is a human A549 cell line in which the genomic SMAD4 gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr
? zinc finger nuclease (ZFN) technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the C-terminus of SMAD4. Fluorescence imaging shows SMAD4-GFP expression throughout the cell. Upon activation with a ligand such as transforming growth factor beta (TGFb), the cell line shows redistribution of SMAD4 from the cytoplasm to the nucleus, making it useful for high content screening to identify compounds that modulate this redistribution. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
Allgemeine Beschreibung
A549 GFP-SMAD4 are lung carcinoma cells from a human 58 year old caucasian male having two distinct ZFN modifications creating two fluorescent transgenes each expressed from their endogenous gene locus.
This cell line was derived from ATCC Catalog No. CCL-185.
A549 Cells GFP-SMAD4 Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
