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ChemicalBook CAS DataBase List Broxuridine
59-14-3

Broxuridine synthesis

9synthesis methods
-

Yield:59-14-3 25%

Reaction Conditions:

with Lactobacillus animalis ATCC 35046 2’-N-deoxyribosyltransferase immobilized in DEAE-Sepharose in aq. buffer at 30; pH=7;Green chemistry;Enzymatic reaction;

Steps:

4.8. Biotransformation of nucleoside analogues

General procedure: Thymidine (dThd) and 2'-deoxyuridine (dUrd) were assayed as sugar donors. Different purine and pyrimidine bases were tested: 5-fluorouracil (5FUra), 5-bromouracil (5BrUra), 5-chlorouracil (5ClUra), 6-chloropurine (6ClPur), 6-bromopurine (6BrPur) and 6-chloro-2-fluoropurine (6Cl2FPur). Reactions were performed using 100 mg/mL of immobilized LaNDT, 6 mM nucleoside and 2 mM base, 30 °C and 200 rpm. At different times (5-8 h), 20 μL aliquots were taken and centrifuged at 10,000 x g, and the supernatant was analyzed by HPLC to evaluate yield expressed as percentage and product conversion expressed as mg of product per gram of support.

References:

Britos, Claudia N.;Lapponi, María José;Cappa, Valeria A.;Rivero, Cintia W.;Trelles, Jorge A. [Journal of Fluorine Chemistry,2016,vol. 186,p. 91 - 96]

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