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The application prospect of luminol luminescence system in different analysis
Release time: 2021-07-14
Luminol has become one of the most widely used chemiluminescent reagents due to its advantages of high quantum yield, easy synthesis, and good water solubility. Its development process is the same as the arduous process of human beings. From the beginning, there is not much understanding, to the current glow and fever, it can only be summarized in one sentence: gold always shines. In the future, there may be more uses we don't know to benefit our lives. We only need to learn more about it to discover its unlimited potential as soon as possible.
Application in the analysis of inorganic substances
Using inorganic ions to enhance or inhibit the chemiluminescence of the luminol system, these metal ions can be measured. It is worth pointing out that some metal ions have higher catalytic activity than metal ions after being combined with complexing agents. For example, using the luminol-H2O2-silver phenanthroline system, the detection limit of silver ions can reach 2.7×10-g/L3.
The use of sensitizers can improve the sensitivity of detection. The sensitization effect of triphenylmethane derivatives on the luminol luminescence system. It was found that Coomassie Brilliant Blue G250 had the best sensitization effect on the luminol-H2O2-Cu2+ system. A reverse flow injection technique was used to establish a measurement of Cu2+. The new method has a detection limit of 1.5μg/mL. The use of the local concentration effect of surfactants to achieve micelle sensitization has been increasingly used in luminescence analysis. For example, the reversed micellar system FIA method is used to measure V4+, the detection limit is 0.05ng/mL; the luminol-vanadium-molybdenum heteropoly acid system is used to measure phosphorus in water samples, and surfactants are used to increase the sensitivity. The limit is reduced by 5 times to 0.02μg/L.
Application in organic matter analysis
The use of organic matter to promote and inhibit the luminol luminescence system can realize the analysis and determination of organic matter. Using N-bromosuccinimide (NBS) and N-chlorosuccinimide (NCS) as oxidants, FIA technology is used to not only determine NBS and NCS in tap water and well water, but also based on cationic surfactants Inhibiting the properties of luminol-NBS (NCS) chemiluminescence, established a new method for the determination of cationic surfactants.
Antioxidants generally inhibit the chemiluminescence of luminol. Using FIA technology, the inhibitory effects of different antioxidants on the luminescence reaction of luminol-active oxygen (superoxide anion O2-, hydroxyl radical OH-, peroxynitrite, H2O2, CIO-) were investigated. Based on the inhibition of antioxidants on the luminol-H2O2 system, combined with HPEC technology, the free radical scavengers in plant extracts were determined. The improvement of this method increased the sensitivity by 4 times. The potential antioxidant cysteine ??has a promoting effect on the luminol-H2O2-Co2+ system, while glutathione has an inhibitory effect, but both have a sensitizing effect on the luminol-pernitrite system. Based on these A new method for the determination of cysteine ??and glutathione has been established.
Application in immunoassay
Another major use of chemiluminescence analysis is immunoassay. The chemiluminescence immunoassay method for labeling luminescent reagents or sensitizer molecules on antigens or antibodies for determination of enzymes, antigens or antibodies has the advantages of safety and pollution-free, stable markers, rapidness, and high sensitivity.
Free-state luminol with high luminous efficiency is often used in enzyme-labeled immunoassays, and the luminescence reaction of luminol with enzyme-labeled sensitization has been used in the determination of vascular endothelial growth factor and testosterone. After labeling the hapten with ferrocene, using its inhibitory effect on the electrochemiluminescence reaction of luminol-H2O2-ferrocene, the hapten and other small molecules were measured, and HRP was labeled on the antibody bound to the liposome. It was found that its activity in catalyzing the luminescence of luminol was 125 times higher than that of HRP directly labeled with antibodies.
Luminol chemiluminescence analysis has been widely used in various fields, and new methods and new technologies are constantly emerging, especially the combination with FIA, HPLC and other technologies, which overcomes the shortcomings of poor selectivity in luminescence analysis and makes the analysis more flexible. Emblem and accuracy. Because of Rumi's stable performance and low price, practical application research and analysis will have broader application prospects.