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International Journal of Biological Macromolecules

International Journal of Biological Macromolecules

IF: 7.7
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Evaluation of cross-linkers in the design of immobilized multi isomerase cascade for the preparation of rare sugars

Published:9 December 2024 DOI: 10.1016/j.ijbiomac.2024.138592 PMID: 39662556
Wenhui Li,?Yuqiu Wu,?Si Long,?Zhi Chen,?Liangzhi Li,?Xin Ju

Abstract

The cascade of sugar isomerases is one of the most practical methods for producing rare sugars, and enzyme immobilization endows it with high economic efficiency, operational convenience and reusability. However, the most employed cross-linker glutaraldehyde (GA) has the disadvantages of enzyme deactivation and limitation of substrate binding. Herein, three compounds, glyoxal, GA, and 2,5-furandicarboxaldehyde (DFF) were evaluated within a previously developed cascade comprising ribose-5-phosphate isomerase and D-tagatose-3-epimerase to prepare D-ribulose form D-xylose. Analyses of surface morphology, element and chemical bond revealed that all compounds effectively cross-linked the isomerases. High concentration of the cross-linkers was generally beneficial for binding protein and preventing enzyme leak during reusing cycles. Glyoxal performed the highest immobilization rate, though it hadn't been employed as a cross-linker for enzyme immobilization. DFF mediated cross-linking revealed the highest activity recovery, substrate conversion and residual activity after reusing cycles, suggesting better biocompatibility than glyoxal and GA. After 8 rounds of recycling, the residual activity of enzyme immobilized by DFF was 61.4?%, ~30?% higher than that of GA. This study proved a potential alternative cross-linker DFF for the immobilization of enzyme cascade with high activity recovery and reusability, which could promote the efficient production of high value-added products from biomass monosaccharides.

Substances (8)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Sodium chloride 7647-14-5 ClNa 1216 suppliers $5.00-$6678.62
Imidazole 288-32-4 C3H4N2 1178 suppliers $5.00-$19203.00
Dopamine hydrochloride 62-31-7 C8H12ClNO2 645 suppliers $16.60-$2055.90
TRIS hydrochloride 1185-53-1 C4H12ClNO3 643 suppliers $7.00-$13159.00
Glyoxal 107-22-2 C2H2O2 634 suppliers $14.00-$1570.00
D(+)-Xylose 58-86-6 C5H10O5 541 suppliers $5.00-$1495.20
Peptone 73049-73-7 C13H24O4 340 suppliers $44.50-$6924.80
KANAMYCIN 59-01-8 C18H36N4O11 194 suppliers $25.00-$1372.96

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