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Chemical Engineering Journal

Chemical Engineering Journal

IF: 13.3
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Simultaneous detection of protein and nucleic acid biomarkers with a CRISPR-based assay

Published:9 December 2024 DOI: 10.1016/j.cej.2024.158452
Yaqin Zhang, Yingjie Cheng, Chenfei Zhao, Jianai Chen, Zhina Wu, Jiasi Wang, Di Wang

Abstract

Simultaneous analysis of multiple protein and nucleic acid biomarkers in a single clinical sample can yield clinically valuable results for diagnosing, surveillance, and managing various diseases. However, the current diagnostic platforms depend largely on protein- or nucleic acid-independent assay, which is time-consuming and laborious. Here, we propose a novel assay termed MCD assay that can simultaneously detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N gene RNA and SARS-CoV-2 S protein. This assay was based on magnetic separation technology, combined with the clustered regularly interspaced palindromic repeats (CRISPR) /CRISPR-associated proteins (Cas) system for accessible point-of-care detection of nucleic acids and proteins. The assay could recognize target molecules through nucleic acid hybridization or antigen–antibody reactions and integrated with the CRISPR/Cas-based enzymatic detection; the lateral flow test strips enabled visualization of the assay results. The limit of detection of MCD was 1000 copies/μL for viral nucleic acid and 10?pg/mL for viral protein. The combined evaluation of multiple biomarkers from diverse pathways can enhance the accuracy and specificity of disease diagnosis. It is also crucial to use a single platform for detecting biomarkers across various dimensions to evaluate and manage major diseases.

Substances (3)

Materials
Procduct Name CAS Molecular Formula Supplier Price
N-Hydroxysuccinimide 6066-82-6 C4H5NO3 886 suppliers $5.00-$3348.80
Tris(2-chloroethyl) phosphate 115-96-8 C6H12Cl3O4P 368 suppliers $19.00-$500.27
DL-Dithiothreitol 27565-41-9 C4H10O2S2 248 suppliers $31.00-$690.00