ACES is the common abbreviation for the compound N-(2-Acetamido)-2-aminoethanesulfonic acid. ACES is a zwitterionic buffer used in biochemistry and molecular biology research. It is one of the Good buffers developed in the 1960s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. ACES is very soluble in water and delivers high clarity in solutions. ACES also works well as a buffer in the isoelectric focusing of proteins. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. This particular product was prepared in 3D water and filtered through a 0.22 ??? filter.

ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe:

Prepare 800 mL of dH2O in a suitable container.

Add 18.22 g of ACES to the solution.

Adjust solution to the desired pH by 10N NaOH.

Add dH2O until the volume is 1 L.