"SN12C Thawing人宮頸癌細(xì)胞系
細(xì)胞背景資料:詳見(jiàn)相關(guān)文獻(xiàn)介紹
細(xì)胞形態(tài):上皮細(xì)胞樣
SN12C Thawing人宮頸癌細(xì)胞系
細(xì)胞生長(zhǎng):貼壁
細(xì)胞常規(guī)培養(yǎng)傳代流程(請(qǐng)嚴(yán)格遵照無(wú)菌操作):1)吸出原培養(yǎng)瓶中的培養(yǎng)基,PBS緩沖液潤(rùn)洗細(xì)胞兩次,加2-3ml 0.25%胰酶進(jìn)行消化細(xì)胞(注意把握消化時(shí)間,通??刂圃冢?2min);2)鏡下觀察消化情況,在細(xì)胞邊緣縮小,貼壁松動(dòng)時(shí)(不建議消化到細(xì)胞漂浮)去掉胰酶,加6-8ml完全培養(yǎng)基,輕輕吹打細(xì)胞層,盡量把細(xì)胞層吹落,吹散;3)取部分細(xì)胞懸液轉(zhuǎn)移到新的培養(yǎng)皿/瓶中,添加適當(dāng)?shù)耐耆囵B(yǎng)基,把細(xì)胞懸液打勻,于培養(yǎng)箱中培養(yǎng);4)注意培養(yǎng)基PH值變化情況,定期換液(每周2-3次),待細(xì)胞密度達(dá)到80%以后重復(fù)1項(xiàng)操作或者凍存;特別注意:(如使用公共實(shí)驗(yàn)室或者初次接觸細(xì)胞培養(yǎng),建議添加雙抗培養(yǎng))1)收到細(xì)胞后請(qǐng)盡快更換為含15%血清的新鮮培養(yǎng)基,如因特殊情況需要繼續(xù)使用原瓶,請(qǐng)?jiān)谠颗囵B(yǎng)基中額外添加10%的血清,(原瓶培養(yǎng)基的繼續(xù)使用時(shí)間最長(zhǎng)不宜超過(guò)72小時(shí));2)貼壁細(xì)胞收到當(dāng)天切忌立刻消化,請(qǐng)將細(xì)胞換液后放置培養(yǎng)箱孵育到第二天再做消化傳代,請(qǐng)優(yōu)先選擇直徑6cm的培養(yǎng)皿進(jìn)行傳代培養(yǎng);3)如簽收時(shí)出現(xiàn)培養(yǎng)瓶壁破裂,漏液等情況請(qǐng)及時(shí)做好照片記錄并聯(lián)系實(shí)驗(yàn)室。
細(xì)胞物種來(lái)源:人源或鼠源等其它物種來(lái)源
細(xì)胞產(chǎn)品包裝:復(fù)蘇形式:T25培養(yǎng)瓶(一瓶)或凍存形式:1ml凍存管(兩支)
細(xì)胞傳代方法:2x10^4 cells/ml
貼壁細(xì)胞消化傳代時(shí)通常采用兩種方法:一、加入胰酶等細(xì)胞脫落后,再加培養(yǎng)基中止胰酶作用,離心傳代;二、加入胰酶后,鏡下觀察待細(xì)胞始脫落時(shí),棄胰酶,加培養(yǎng)液分瓶。但前者太麻煩,而后者有可能對(duì)細(xì)胞施加胰酶選擇,因?yàn)榭偸琴N壁不牢的細(xì)胞先脫落,對(duì)腫瘤細(xì)胞來(lái)說(shuō),這部分細(xì)胞有可能是惡性程度較高的細(xì)胞亞群。一種簡(jiǎn)單的消化傳代方法。加入PBS洗去血清或加入胰酶先中和血清的作用(30s),棄之,再加入適量胰酶作用10s-40s(根據(jù)細(xì)胞消化的難易程度),棄之,這樣依賴殘余的胰酶就可將細(xì)胞消化單細(xì)胞。對(duì)于較難消化的細(xì)胞,可以用2%利多卡因消化5-8分鐘,然后再棄去,加培養(yǎng)基吹打也可以,對(duì)細(xì)胞的影響不大。不用PBS也不用Hanks洗,只要把舊培養(yǎng)液吸的干凈一點(diǎn),直接加酶消化應(yīng)該不會(huì)有什么問(wèn)題。棄培養(yǎng)液后,用0.04%的EDTA沖洗一次,再用1/4v的0.04%的EDTA室溫孵育5min,棄取大部分EDTA,加入與剩余EDTA等量的胰酶(預(yù)熱)總體積1/10v。消化到有細(xì)胞脫落。不過(guò)有人說(shuō)EDTA對(duì)細(xì)胞不好,有證據(jù)嗎?培養(yǎng)的BASMC:倒掉舊培養(yǎng)液加入少量胰酶沖一下,倒掉再加入0.125-0.25%胰酶約6-10滴或1ml(25ml bottle)消化再加入適量新培養(yǎng)基中和,并分瓶這種方法簡(jiǎn)單、省事;效果很好并且不損失細(xì)胞!
細(xì)胞來(lái)源說(shuō)明:細(xì)胞主要來(lái)源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少數(shù)國(guó)外著名大學(xué)建系
SN12C Thawing人宮頸癌細(xì)胞系
細(xì)胞生長(zhǎng)特性:貼壁生長(zhǎng)
血清必須貯存于–20~-70℃,若存放于4℃,請(qǐng)勿超過(guò)一個(gè)月。如果一次無(wú)法用完一瓶,可將40~45ml分裝于無(wú)菌50ml離心管中,由于血清結(jié)凍時(shí)體積會(huì)增加約10%,必須預(yù)留此膨脹體積之空間,否則易發(fā)生污染或容器凍裂之情形。一般廠商提供之血清為無(wú)菌,不需再無(wú)菌過(guò)濾。若發(fā)現(xiàn)血清有許多懸浮物,則可將血清加入培養(yǎng)基內(nèi)一起過(guò)濾,勿直接過(guò)濾血清。瓶裝(500ml)血清解凍步驟(逐步解凍法):-20℃或–70℃至4℃冰箱溶解一天,至室溫下全溶后再分裝,一般以50ml無(wú)菌離心管可分裝40~45ml。在溶解過(guò)程中須規(guī)則搖晃均勻(小心勿造成氣泡),使溫度與成分均一,減少沈淀的發(fā)生。勿直接由–20℃直接至37℃解凍,因溫度改變太大,容易造成蛋白質(zhì)凝結(jié)而發(fā)生沈淀。勿將血清置于37℃太久,若在37℃放置太久,血清會(huì)變得混濁,同時(shí)血清中許多較不穩(wěn)定之成份亦會(huì)因此受到破壞,而影響血清之品質(zhì)。血清凝絮物:發(fā)生之原因有許多種,但普遍之原因是血清中之脂蛋白(lipoprotein) 變性及解凍后血清中存在之血纖維蛋白(fibrin) 造成,這些凝絮沈淀物不會(huì)影響血清本身之品質(zhì)。若欲減少這些凝絮沈淀物,可用離心3000rpm,5min去除,或離心后上清液可以加入培養(yǎng)基中一起過(guò)濾。不建議用過(guò)濾步驟去除這些凝絮沈淀物,因?yàn)闀?huì)阻塞過(guò)濾膜。顯微鏡下觀察之“小黑點(diǎn)”:通常經(jīng)過(guò)熱處理之血清,沈淀物的形成會(huì)顯著的增多。有些沈淀物在顯微鏡下觀察像是“小黑點(diǎn)”,常會(huì)誤認(rèn)為血清遭受污染,而將血清放在37℃中欲培養(yǎng)此“微生物”,但在37℃環(huán)境下,又會(huì)使此沈淀物增多,更會(huì)誤認(rèn)為微生物之增殖,但以培養(yǎng)細(xì)菌之培養(yǎng)基檢測(cè),又沒(méi)有污染。一般而言,此小黑點(diǎn)應(yīng)不會(huì)影響細(xì)胞之生長(zhǎng),但若懷疑此血清之品質(zhì),應(yīng)立即停用,更換另一批號(hào)的血清。
細(xì)胞形態(tài)特性:上皮細(xì)胞樣
H1435細(xì)胞系相關(guān)產(chǎn)品:Panc5.04細(xì)胞、NCIH1395細(xì)胞、190PT細(xì)胞
149 PT細(xì)胞系相關(guān)產(chǎn)品:Hs 578T細(xì)胞、H-9細(xì)胞、SNU878細(xì)胞
SACCLM細(xì)胞系相關(guān)產(chǎn)品:HEK 293細(xì)胞、HCT/Taxo1細(xì)胞、Det. 562細(xì)胞
OUMS-27細(xì)胞系相關(guān)產(chǎn)品:KYSE270細(xì)胞、H774細(xì)胞、B-cell Acute Lymphoblastic Leukemia-1細(xì)胞
A-172細(xì)胞系相關(guān)產(chǎn)品:SK Mel 2細(xì)胞、HPAF-2細(xì)胞、KALS-1細(xì)胞
Centre Antoine Lacassagne-33細(xì)胞系相關(guān)產(chǎn)品:Det. 562細(xì)胞、MV522細(xì)胞、MAC1細(xì)胞
SW954細(xì)胞系相關(guān)產(chǎn)品:KNS81細(xì)胞、MIA-Pa-Ca-2細(xì)胞、HCC15細(xì)胞
DHL-5細(xì)胞系相關(guān)產(chǎn)品:KS-1 [Human glioblastoma]細(xì)胞、Human Microvascular Endothelial Cell line-1細(xì)胞、HCC0070細(xì)胞
293 F細(xì)胞系相關(guān)產(chǎn)品:Huh-7.5.1細(xì)胞、RS4-11細(xì)胞、U87細(xì)胞
KCL-22細(xì)胞系相關(guān)產(chǎn)品:Central Adrenergic TH-expressing a細(xì)胞、MKN28細(xì)胞、SaOS細(xì)胞
PLCPRF5細(xì)胞系相關(guān)產(chǎn)品:SCL-II細(xì)胞、QBC939細(xì)胞、L929(NCTC)細(xì)胞
C6細(xì)胞系相關(guān)產(chǎn)品:NCIH1975細(xì)胞、GNM細(xì)胞、MUTZ-3細(xì)胞
MPC-83細(xì)胞系相關(guān)產(chǎn)品:PL9細(xì)胞、MC 116細(xì)胞、H1355細(xì)胞
H1954細(xì)胞系相關(guān)產(chǎn)品:Eca109細(xì)胞、HEK-293-FT細(xì)胞、EAC細(xì)胞
Jeko1細(xì)胞系相關(guān)產(chǎn)品:Mel-RM細(xì)胞、NCI-H676細(xì)胞、SHZ-88細(xì)胞
Mel624細(xì)胞系相關(guān)產(chǎn)品:NCIH847細(xì)胞、CCD 841 CoN細(xì)胞、Functional Liver Cell-7細(xì)胞
SW900細(xì)胞系相關(guān)產(chǎn)品:NCI-H510細(xì)胞、HCA7細(xì)胞、BxPC-3細(xì)胞
LCD細(xì)胞系相關(guān)產(chǎn)品:NCI-SNU-520細(xì)胞、ISHI細(xì)胞、GM12878細(xì)胞
SEG1細(xì)胞系相關(guān)產(chǎn)品:NCI H929細(xì)胞、MDA-435細(xì)胞、Jurkat E6細(xì)胞
MD Anderson-Metastatic Breast-330細(xì)胞系相關(guān)產(chǎn)品:RINm-5F細(xì)胞、3T3-Swiss albino細(xì)胞、EBC1細(xì)胞
Doubling time: ~50 hours (ATCC).細(xì)胞系相關(guān)產(chǎn)品:OV1/P細(xì)胞、HFL細(xì)胞、RBSMC細(xì)胞
ADR-RES細(xì)胞系相關(guān)產(chǎn)品:SG231細(xì)胞、B-16細(xì)胞、Hs600T細(xì)胞
3AO細(xì)胞系相關(guān)產(chǎn)品:TH1細(xì)胞、H711細(xì)胞、RN細(xì)胞
MC3T3細(xì)胞系相關(guān)產(chǎn)品:P3-NS1/1-Ag4-1細(xì)胞、Panc 02細(xì)胞、COLO 201細(xì)胞
MADISON LUNG TA-109細(xì)胞系相關(guān)產(chǎn)品:PK136細(xì)胞、MIA PaCa-2細(xì)胞、C666-1細(xì)胞
H-EMC-SS細(xì)胞系相關(guān)產(chǎn)品:BSC-40細(xì)胞、HS 683T細(xì)胞、U-373 MG細(xì)胞
SN12C Thawing人宮頸癌細(xì)胞系
H-2444細(xì)胞系相關(guān)產(chǎn)品:CAL85-1細(xì)胞、PA-1細(xì)胞、NCI-H1341細(xì)胞
OS-RC-2細(xì)胞系相關(guān)產(chǎn)品:NUGC2細(xì)胞、SF-767細(xì)胞、3T6細(xì)胞
RINm-5F細(xì)胞系相關(guān)產(chǎn)品:Capan-2細(xì)胞、NCIH1781細(xì)胞、hs 68細(xì)胞
HCoEpiC細(xì)胞系相關(guān)產(chǎn)品:P36細(xì)胞、C8D1A細(xì)胞、HONE-1細(xì)胞
A2008細(xì)胞系相關(guān)產(chǎn)品:OsACL細(xì)胞、AU-Mel細(xì)胞、H-35 Reuber細(xì)胞
CWR22Rv1細(xì)胞系相關(guān)產(chǎn)品:NCI-H2110細(xì)胞、Det 562細(xì)胞、HME-1細(xì)胞
QBC-939細(xì)胞系相關(guān)產(chǎn)品:RC13細(xì)胞、TSUpr1細(xì)胞、P31/Fujioka細(xì)胞
COLO 320F細(xì)胞系相關(guān)產(chǎn)品:G-402細(xì)胞、IAR-20細(xì)胞、Immortalized Human Hepatocytes細(xì)胞
OCI-Ly 10細(xì)胞系相關(guān)產(chǎn)品:IEC 18細(xì)胞、HLE B-3細(xì)胞、WILL-2細(xì)胞
ST2細(xì)胞系相關(guān)產(chǎn)品:R D細(xì)胞、RS4:11細(xì)胞、Hs675細(xì)胞
SNU398細(xì)胞系相關(guān)產(chǎn)品:G 401細(xì)胞、SKUT-1細(xì)胞、SK-N-BE(2)-C細(xì)胞
Strain L-929細(xì)胞系相關(guān)產(chǎn)品:LED-WiDr細(xì)胞、H1915細(xì)胞、MT-4細(xì)胞
MSCs(mUCMSCs)細(xì)胞系相關(guān)產(chǎn)品:NCIH64細(xì)胞、NIH:OVCAR4細(xì)胞、MD Anderson-Metastatic Breast-231細(xì)胞
H-1417細(xì)胞系相關(guān)產(chǎn)品:CFPAC1細(xì)胞、NCIH1581細(xì)胞、KOSC-2 cl3-43細(xì)胞
Toledo細(xì)胞系相關(guān)產(chǎn)品:G-361細(xì)胞、H-295細(xì)胞、KP4細(xì)胞
LLC-PK-1細(xì)胞系相關(guān)產(chǎn)品:KHYG細(xì)胞、INS-1細(xì)胞、HEK293EBNA細(xì)胞
KMS11細(xì)胞系相關(guān)產(chǎn)品:B 95-8細(xì)胞、HCA-7細(xì)胞、RBMEC細(xì)胞
HUT-28細(xì)胞系相關(guān)產(chǎn)品:P-815細(xì)胞、Hut292細(xì)胞、NMCG1細(xì)胞
HSC-I細(xì)胞系相關(guān)產(chǎn)品:NCI-H69細(xì)胞、Co 205細(xì)胞、HKC細(xì)胞
Tu 177細(xì)胞系相關(guān)產(chǎn)品:KP-4細(xì)胞、NCI-SNU-1細(xì)胞、H-82細(xì)胞
Huh-7.5細(xì)胞系相關(guān)產(chǎn)品:HEK 293-H細(xì)胞、NCI-H510細(xì)胞、MA104細(xì)胞
MDAMB436細(xì)胞系相關(guān)產(chǎn)品:SW-1990細(xì)胞、UMR-106細(xì)胞、SJRH-30細(xì)胞
Colo-201細(xì)胞系相關(guān)產(chǎn)品:NK-92 MI細(xì)胞、BEL 7404細(xì)胞、Ha Fe細(xì)胞
GM03570細(xì)胞系相關(guān)產(chǎn)品:Onda 11細(xì)胞、NIHOVCAR3細(xì)胞、Mouse Bladder Tumor line-2細(xì)胞
LoVo細(xì)胞系相關(guān)產(chǎn)品:KYSE 270細(xì)胞、SNU878細(xì)胞、NCI-H1105細(xì)胞
KNS42細(xì)胞系相關(guān)產(chǎn)品:MV4II細(xì)胞、HCC0095細(xì)胞、NCI-H526細(xì)胞
C-127細(xì)胞系相關(guān)產(chǎn)品:McARH7777細(xì)胞、TOV112細(xì)胞、BPH-1細(xì)胞
SW1353細(xì)胞系相關(guān)產(chǎn)品:GP293細(xì)胞、L5178Y細(xì)胞、CAL-51細(xì)胞
G-292細(xì)胞系相關(guān)產(chǎn)品:U-CH1細(xì)胞、NCTC clone 1469細(xì)胞、H-647細(xì)胞
HBE 135-E6E7細(xì)胞系相關(guān)產(chǎn)品:SaOS細(xì)胞、HPAEpiC細(xì)胞、Clone 929細(xì)胞
Michigan Cancer Foundation-10A細(xì)胞系相關(guān)產(chǎn)品:ST486細(xì)胞、VP303細(xì)胞、3T3-J2細(xì)胞
Turbot Embryonic Cell line細(xì)胞系相關(guān)產(chǎn)品:RAOEC細(xì)胞、SKNEP細(xì)胞、Caco-2/BBe細(xì)胞
TALL1細(xì)胞系相關(guān)產(chǎn)品:VERO76細(xì)胞、293-H細(xì)胞、SHZ-88細(xì)胞
SN12C Thawing人宮頸癌細(xì)胞系
Y-79細(xì)胞系相關(guān)產(chǎn)品:MBT2細(xì)胞、DITNC1細(xì)胞、CX-1細(xì)胞
CCD 1112SK細(xì)胞系相關(guān)產(chǎn)品:OVCAR-432細(xì)胞、OV1-P細(xì)胞、H-1650細(xì)胞
Hs870T細(xì)胞系相關(guān)產(chǎn)品:JG細(xì)胞、NCI-H748細(xì)胞、SK-MEL-1細(xì)胞
MAEC細(xì)胞系相關(guān)產(chǎn)品:NCIH820細(xì)胞、TF1細(xì)胞、RBL細(xì)胞
K7M2 wt細(xì)胞系相關(guān)產(chǎn)品:Caov-4細(xì)胞、J-774A.1細(xì)胞、Jeko1細(xì)胞
HFLS-OA細(xì)胞系相關(guān)產(chǎn)品:HFF-1細(xì)胞、L5178-R細(xì)胞、COV 434細(xì)胞
PAN 02細(xì)胞系相關(guān)產(chǎn)品:MFM-223細(xì)胞、MTEC1細(xì)胞、RAW-264.7細(xì)胞
alpha TC1-6細(xì)胞系相關(guān)產(chǎn)品:Namalva細(xì)胞、H-1688細(xì)胞、NCIH2228細(xì)胞
NCI-H2198細(xì)胞系相關(guān)產(chǎn)品:Ca761細(xì)胞、OVCAR5細(xì)胞、DSL6A/C1細(xì)胞
MDA-MB-415細(xì)胞系相關(guān)產(chǎn)品:NCI.H23細(xì)胞、QSG7701細(xì)胞、H-1930細(xì)胞
NCIH650細(xì)胞系相關(guān)產(chǎn)品:K7M2 wt細(xì)胞、T 24細(xì)胞、MDA-361細(xì)胞
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