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Annexin V-APC/7-AAD 凋亡試劑盒,Annexin V-APC/7-AAD apoptosis analysis kit
  • Annexin V-APC/7-AAD 凋亡試劑盒,Annexin V-APC/7-AAD apoptosis analysis kit

Annexin V-APC/7-AAD 凋亡試劑盒

價格 詢價
包裝 25T 100T
最小起訂量 25T
發(fā)貨地 上海
更新日期 2023-10-16

產(chǎn)品詳情

中文名稱:Annexin V-APC/7-AAD 凋亡試劑盒英文名稱:Annexin V-APC/7-AAD apoptosis analysis kit
產(chǎn)品類別: 常規(guī)試劑
2023-10-16 Annexin V-APC/7-AAD 凋亡試劑盒 Annexin V-APC/7-AAD apoptosis analysis kit 25T/RMB;100T/RMB 常規(guī)試劑
描述

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining.

Products List:Annexin V-APC、7-AAD Viability Staining solution、Annexin V Binding Buffer、Apoptosis Positive Control Solution.

 
應(yīng)用
Each lot of this reagent is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used 5 µl/10^5 cells). Since applications vary, the appropriate dilutions must be determined for individual use.
使用方法
Suggested Staining Protocol:
A.Parameters regulation
1. Harvest cell(1×106-3×106cells), then sepatate the cells in two parts. Wash cells with cold PBS, then centrifuge the cells and disgard the supernatant.
2. Suspend one part of cells in 200μL 1× binding buffer, store at 4℃ for use.
3. Suspend the other part of cells in 500μL Apoptosis Positive Control Solution, and incubate for 10 minutes in room temperature. Wash cells with more than 3.0 mL cold PBS, blot the supernatant, then suspend the cells in 200μL 1× binding buffer.
4. Mix the two parts cells together, then separate the cells in three tubes, and add 100 μL of cells in each tube.
5. The first tube is Blank Control, the second one adds 5μL of Annexin V-APC, and the third one adds 5 μL 7-AAD solution.
6. Gently vortex each tube and incubate for 5 minutes in room temperature, protected from light.
7. Before analyzing by flow cytometry, using the blank control and single dye sample to regulate voltage and compensation, as shown in Figure Parameters regulation.
B.Sample detection
1. Dilute 6 mL 5× binding buffer with 24 mL distilled water for 10 tests.
2. Harvest cell(about 1×106cells per test)then wash with cold PBS.
3. Suspend cells in 1 mL 1× Binding Buffer, 300×g centrifugation for 10 minutes, then remove the Binding Buffer from the cell pellet.
4. Resuspend cells in 1 mL 1×Binding Buffer , adjust cell concentration to 1×106cells/mL.
5. Add 100 μL of cells (1×105cells) to each labeled tube.
6. Add 5 μL of Annexin V-APC to appropriate tubes.
7. Gently vortex each tube and incubate for 10 minutes in room temperature, protected from light.
8. Add 5 μL 7-AAD solution incubation for 5min in room temperature, protected from light.
9. Add PBS to 500μL and vortex gently.
10. Analyze by flow cytometry in 1 hour
關(guān)鍵字: Annexin V-APC/7-AAD 凋亡試劑盒;Annexin V-APC/7-AAD apoptosis analysis kit

公司簡介

愛必信(上海)生物科技有限公司成立于2010年,位于上海市申江科創(chuàng)園。是一家自主研發(fā)和生產(chǎn)生物試劑的公司。 Absin?是愛必信(上海)生物科技有限公司的試劑品牌,目前已近60萬種生化試劑,10萬種抗體,其基礎(chǔ)生化試劑在基礎(chǔ)科學(xué)領(lǐng)域得到廣泛應(yīng)用的同時,特色產(chǎn)品線常規(guī)生化試劑,血清,小分子激動劑及抑制劑在業(yè)界也備受好評。 Absin秉承踏實,務(wù)實,求精和不斷創(chuàng)新的精神為中國科學(xué)家提供最好的產(chǎn)品,努力打造屬于中國自己的生物試劑品牌,為廣大科學(xué)家提供更全面更豐富更優(yōu)質(zhì)的產(chǎn)品。 愛科研,必信它(Absin)!Absin努力成為你身邊的科研百寶箱!
成立日期 2010-12-13 (15年) 注冊資本 2500萬
員工人數(shù) 100-500人 年營業(yè)額 ¥ 1000萬-5000萬
主營行業(yè) 生化試劑,抗體,分子生物學(xué),生物活性小分子 經(jīng)營模式 工廠
  • 愛必信(上海)生物科技有限公司
非會員
  • 公司成立:15年
  • 注冊資本:2500萬
  • 企業(yè)類型:有限責(zé)任公司
  • 主營產(chǎn)品:二抗、標(biāo)簽抗體、對照抗體;WB相關(guān):ECL發(fā)光液、預(yù)染marker、預(yù)制膠;IHC相關(guān):二抗試劑盒、組化筆;IP/CoIP試劑盒;細(xì)胞因子、信號通路調(diào)節(jié)劑;血清、BSA、蛋白酶K、CTB、TTX、CEE;凋亡試劑盒、呼吸爆發(fā)試劑盒;動植物提取物,定制服務(wù)(抗體/多肽/蛋白)...
  • 公司地址:上海市浦東新區(qū)新浩路58號18號樓
詢盤

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