細胞自噬是真核生物在保守進化中對細胞內(nèi)物質(zhì)進行周轉(zhuǎn)的重要過程，該過程中一些損壞的蛋白或細胞器被雙層膜結(jié)構(gòu)的自噬小泡包裹后，送入溶酶體中進行降解并得以循環(huán)利用。動物細胞衰老反應(yīng)過程中，往往發(fā)生分子伴侶介導(dǎo)的自噬過程，細胞自噬與細胞凋亡、細胞衰老一樣，是十分重要的生物學現(xiàn)象，它參與生物的發(fā)育、生長等多種過程，細胞自噬的異常易導(dǎo)致癌細胞的出現(xiàn)。ProSci有很多種細胞自噬抗體，其中包括自噬損害調(diào)節(jié)器DRAM抗體和自噬相關(guān)蛋白APG7、PIST、LAMP-1、LAMP-2抗體。此外，ProSci還提供自噬檢測試劑盒。ProSci的每種自噬抗體都可適用于ELISA，western blot和免疫組織化學（IHC）。
Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Description
Western blot analysis of Beclin-1 in 293 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of beclin-1 in rat brain tissue with beclin-1 antibody at 2 μg/mL.
Additional Names
Beclin-1 Antibody: Beclin-1, Coiled-coil myosin-like Bcl-2-interacting protein
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Formulation
Physical State: Liquid
Concentration: 1 mg/mL
Isotype
IgG
Clonality/Clone
This is a polyclonal Beclin-1 Antibody.
Conjugate
None
Purification
Beclin-1 Antibody is affinity chromatography purified via peptide column.
Host
Beclin-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Beclin-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1.
Application
Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. Tested Application
E, WB, IHC
Buffer
Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3613P - Beclin-1 Peptide
Storage
Beclin-1 antibody can be stored at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Positive Control
- Cat. No. 1210 - 293 Cell Lysate
- Cat. No. 1403 - Mouse Brain Tissue Lysate]
Species Reactivity
H, M, R
Protein GI Number
16307457
Protein Accession Number
AAH10276 Reference
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
- Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.]