名稱(chēng) Ponceau S 麗春紅S
其它名稱(chēng)
英文名稱(chēng)：Ponceau S；C.I. 27195；3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid tetrasodium salt其他名稱(chēng)：猩紅S；酸性紅112；3-羥基-4-[2-磺酸基-4-(4-磺酸苯基偶氮)苯基偶氮]-2,7-萘二磺酸四鈉鹽
規(guī)格
PH1428-10 | 10g
產(chǎn)品簡(jiǎn)介
Ponceau S
CAS Number: 6226-79-5
Storage Temperature: Room Temperature
Product Description :
Appearance: Deep red crystalline powder
Molecular formula: C22H12N4Na4O13S4
Molecular weight: 760.6
Ponceau S is a red, water-soluble, anionic diazo dye that is made by coupling diazotized 4-amino-1,1’-azobenzene-3,4’-disulfonic acid (Acid Yellow 9) with 2-naphthol-3,6-disulfonic acid(R acid)and converting the coupled product to the tetrasodium salt.
Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and is widely used in gel electrophoresis. It also binds non-covalently to non-polar regions in the protein.
Ponceau S may not be as sensitive of a protein stain as Coomassie Blue. It is superior to Acid Fuchsin in the VanGieson stain.
Preparation Instructions :
It is soluble in water (1 mg/ml), yielding a clear solution.
Procedure:
A solution as low as 1% ponceau S can be made in 7% acetic acid (w/w) for staining polyacrylamide gels. The gel can be left in this solution for eight hours. However, prior to placing the gel in this staining mixture, the proteins on the gels should be fixed with a fixative agent such as methanol:acetic acid or TCA.
Destaining of the gel can occur in 7% (v/v) acetic acid:water mixture with many changes of the destaining solution.
Storage/Stability:
Recommended storage is at room temperature. The shelf-life should be at least two years.
Precautions and Disclaimer :
For Laboratory Use Only. Not for drug, household or other uses.
關(guān)鍵字： 麗春紅S，麗春紅S價(jià)格，麗春紅S現(xiàn)貨
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