"MDA-MB-231人乳腺癌細胞全年復蘇|已有STR圖譜

傳代比例：1:2-1:4(首次傳代建議1:2)

生長特性：貼壁生長

細胞系的應用：1)免疫組化研究2)RNA干擾研究3)藥物作用研究4)慢病毒轉(zhuǎn)染研究等其它應用。細胞系通常用于實驗研究，如增殖、遷移、侵襲等。細胞系在多個領(lǐng)域的研究中被廣泛應用，包括基礎(chǔ)醫(yī)學、臨床試驗、藥物篩選和分子生物學研究。這些研究不僅在中國，也在日本、美國和歐洲等多個國家和地區(qū)進行。

換液周期：每周2-3次

U2OS Cells;背景說明：骨肉瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hela-Ap-1細胞、BIU-87細胞、KLN-205細胞

CEMO-1 Cells;背景說明：急性B淋巴細胞白血??；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HCC-1395細胞、MADB 106細胞、NUGC-3細胞

L6 Cells;背景說明：該細胞是Yaffe在甲基膽蒽存在的情況下從大鼠大腿肌原代培養(yǎng)的最初兩代細胞中分離得到的；在培養(yǎng)基中融合形成多核的肌管和橫紋肌纖維，細胞融合的程度隨著代數(shù)的增加而下降，因此細胞應低代次冷凍并周期性地重新克隆以選擇融合能力強的細胞。鼠痘病毒陰性。該細胞應在達匯合狀態(tài)前傳代，以延緩細胞分化能力的喪失。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：成肌細胞樣;相關(guān)產(chǎn)品有：SKCO 1細胞、DU4475細胞、Michigan Cancer Foundation-12F細胞

背景信息：-MB-２３１來自患有轉(zhuǎn)移乳腺腺癌的５１歲女病人的胸水。在裸鼠和ALS處理的BALB/c小鼠中，它能形成低分化腺癌（III級）。

MDA-MB-231人乳腺癌細胞全年復蘇|已有STR圖譜

產(chǎn)品包裝：復蘇發(fā)貨：T25培養(yǎng)瓶(一瓶)或凍存發(fā)貨：1ml凍存管(兩支)

公司細胞系主要引進ATCC、DSMZ、JCRB、KCLB、RIKEN、ECACC等細胞庫，細胞系體外培養(yǎng)，它們會成長為單層細胞，附著或緊貼在培養(yǎng)瓶上，或懸浮在體外的溶液中，細胞系復蘇周期短，公司細胞系狀態(tài)良好，飽滿，有光澤等優(yōu)點。EDTA的作用：許多人不用胰酶，只用EDTA，或者用胰酶/EDTA聯(lián)合作用。這里要明白，胰酶切割細胞外基質(zhì)的一些負責粘連和附著的蛋白，而EDTA通過螯合Ca離子，作用于整聯(lián)蛋白的活性，所以EDTA的作用更加溫和。有的人在胰酶里添加一些EDTA，或者對付特別難消化的細胞，添加多一些EDTA，就是這個道理。一般不要試圖延長消化時間(如果10min還消化不下來的話)，而應該想其它辦法。

HEK 293A Cells;背景說明：胚腎；腺病毒包裝;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：ESC-410細胞、LC-1/sq細胞、RGC5細胞

NCI/ADR-RES Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：FM-88細胞、DC 2.4細胞、L-cell細胞

GM637 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：OCI-Ly19細胞、DMS273細胞、H865細胞

45.6.3.1 Cells(提供STR鑒定圖譜)

來源說明：細胞主要來源ATCC、ECACC、DSMZ、RIKEN等細胞庫

物種來源：人源、鼠源等其它物種來源

MDA-MB-231人乳腺癌細胞全年復蘇|已有STR圖譜

形態(tài)特性：上皮細胞樣

細胞復蘇相關(guān)注意事項：１.取細胞的過程中注意帶HAO防凍手套，護目鏡。此項尤為重要，細胞凍存管可能漏入，解凍時凍存管中的氣溫急劇上升，可導致爆炸。２.凍存的問題：凍存的配置已是常識，在這里不作詳述，但二甲基亞砜(DMSO )對細胞不是完全無毒副作用，在常溫下，二甲基亞砜對細胞的毒副作較大，因此，必須在１-２min內(nèi)使凍存完全融化。如果復蘇溫度太慢，會造成細胞的損傷，二甲基亞砜(DMSO)ZuiHAO選擇進口產(chǎn)品。３.離心前須加入少量培養(yǎng)。細胞解凍后二甲基亞砜濃度較GAO，注意加入少量培養(yǎng)可稀釋其濃度，以減少對細胞的損傷。４.離心問題：目前主要有兩種見解。一種是解凍后的細胞懸直接吹打均勻后分裝到培養(yǎng)瓶中進行培養(yǎng)，第二天換。因為離心的目的是兩個，去除DMSO，去除死細胞，這個是標準流程，但對一般人來說，把握不HAO離心轉(zhuǎn)速和時間，轉(zhuǎn)的不夠活細胞沉底的少，細胞就全被扔掉了，轉(zhuǎn)過了活細胞會受壓過大，死亡。此外在操作過程中容易污染，所以不推薦。另一種說法為細胞懸中含有二甲基亞砜(DMSO)，DMSO對細胞有一定的毒副作用，所以須將離心后的體前倒凈，且一定倒干凈。我在試驗中按照常規(guī)的離心分裝的方法進行復蘇，結(jié)果無異常。５.細胞貼壁少的問題：教科書中說明凍存細胞解凍時１ml細胞要加１０ml-１５ml培養(yǎng)，而在我的試驗中的經(jīng)驗總結(jié)為培養(yǎng)基越少細胞越容易貼附。６.復蘇細胞分裝的問題：試驗中我的經(jīng)驗總結(jié)為復蘇１管細胞一般可分裝到１-２只培養(yǎng)瓶中，分裝過多，細胞濃度過低，不利于細胞的貼壁。７.加培養(yǎng)基的量放入問題：這個量的多少的把握主要涉及到的問題DMSO的濃度，從如果你加培養(yǎng)基的太少，那么DMSO的濃度就會比較大，就會影響細胞生長，從以前的資料來看，DMSO的濃度在小于０.５%的時候?qū)σ话慵毎麤]有什么影響，還有一個說法是１%。所以如果你的凍存的濃度是１０%DMSO的話那么加１０ml以上的培養(yǎng)基就恰HAO稀釋到了無害濃度。

H526 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：每周換液２-３次。;生長特性：懸浮生長 ;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：PLCPRF5細胞、HMy2.CIR細胞、GTL-16細胞

SGC-996 Cells;背景說明：膽囊癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Hs 604.T細胞、HCET細胞、LAN1細胞

NCI H508 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NB9細胞、NK-92.05細胞、J45.01細胞

H522 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３-１：６傳代；每周換液２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：HUASMC細胞、JHH-2細胞、Daudi細胞

NKT Cells;背景說明：NK Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MASMC細胞、Tca8113細胞、SKMEL5細胞

HuT-78 Cells;背景說明：皮膚；T淋巴瘤；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：LLC-PK1細胞、University of Arizona Cell Culture-812細胞、LS-513細胞

NU-GC-3 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：６傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：MGC803細胞、ACCM細胞、MC 116細胞

HA1800 Cells;背景說明：星形膠質(zhì) Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HUSMC細胞、SUDHL-10細胞、K7M2-WT細胞

WEHI-231 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H2170細胞、CCC-HPF-1細胞、SW-900細胞

hRMECs Cells;背景說明：視網(wǎng)膜微血管；內(nèi)皮 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HSAS4細胞、LSECs細胞、NCI-H1672細胞

alpha TC1.6 Cells;背景說明：胰島素瘤；a細胞；C５７BL/６xDBA/２;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HO1N1細胞、MIN-6細胞、BC-024細胞

RFL-6 Cells;背景說明：胚肺；成纖維細胞；SD大鼠;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SNUC2B細胞、PG13細胞、Stanford University-Diffuse Histiocytic Lymphoma-8細胞

DHL-6 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３—１：６傳代，３—４天換液１次;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：MLMA細胞、H719細胞、A549/DDP細胞

GTL 16 Cells;背景說明：胃癌；肝轉(zhuǎn)移；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：FO [Mouse myeloma]細胞、UPCISCC154細胞、MDAMB436細胞

CHP 126 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-23細胞、OVCAR.3細胞、Cates-1B細胞

C-Li-7 Cells;背景說明：人肝癌細胞株。這株細胞從裸鼠體外移植瘤中建立。;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SW-780細胞、SJSA1細胞、KU 812F細胞

C4-2 Bone metastatic Cells;背景說明：前列腺癌；左鎖骨上淋巴結(jié)轉(zhuǎn)移；男性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H-82細胞、SW-527細胞、NBL-7細胞

68-41-Cas9 Cells(提供STR鑒定圖譜)

Abcam MCF-7 B2M KO Cells(提供STR鑒定圖譜)

ARMD1-hiPSC5 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line RRM201 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line XST061 Cells(提供STR鑒定圖譜)

CARNAVAL Cells(提供STR鑒定圖譜)

DA00809 Cells(提供STR鑒定圖譜)

DD2606 Cells(提供STR鑒定圖譜)

GM02162 Cells(提供STR鑒定圖譜)

BJ [Human fibroblast] Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：成纖維細胞樣;相關(guān)產(chǎn)品有：H-2591細胞、PGBE1細胞、JCA-1細胞

Hep2 Cells;背景說明：最初認為這個細胞源自喉上皮癌，但隨后通過同功酶分析、HeLa標記染色體和DNA指紋分析發(fā)現(xiàn)，起源細胞已被HeLa污染。 角蛋白免疫過氧化物酶染色陽性。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：H-660細胞、hFOB1.19細胞、LICR-LON-HN6-R細胞

Menschliche Und Tierische Zellkulture-1 Cells;背景說明：骨髓增生異常綜合征；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCIH1838細胞、H157細胞、SK 1細胞

KS-1 [Human glioblastoma] Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：成纖維細胞;相關(guān)產(chǎn)品有：BEL 7405細胞、BC-021細胞、TE-7細胞

UWB1289 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：A 2780 CP細胞、Panc_05_04細胞、NCIH2081細胞

SVOG Cells;背景說明：卵巢；顆粒 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Panc-1-P細胞、HCC94細胞、B10BR細胞

hTERTHME1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：SK-MEL-3細胞、PaCa2細胞、LY-R細胞

Calu-6 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：消化２０分鐘。１：２。５-６天長滿。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：GTL-16細胞、SW-13細胞、GM06141細胞

MDA-MB-231人乳腺癌細胞全年復蘇|已有STR圖譜

P3-X63-Ag8-653 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H250細胞、JKT1細胞、SJSA細胞

SW 1116 Cells;背景說明：CSAp陰性(CSAp-)。 結(jié)腸抗原３，陰性。 角蛋白免疫過氧化物酶染色陽性。 癌基因c-myc, K-ras, H-ras, myb, sis 和fos的表達呈陽性。 未檢測到癌基因N-myc和N-ras的表達。 表達腫瘤特異的核基質(zhì)蛋白CC-４，CC-５和CC-６。;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：WM239A細胞、PF382細胞、MCF-12A細胞

NK-10A Cells;背景說明：１９６７年，該細胞系KleinE和KleinG建系，源于一名１６歲患有Burkitt＇s淋巴瘤的黑人男性，beta-２-微球蛋白陰性，表達EBNA,VCA，sIg。該細胞攜帶EB病毒，是一個典型的B淋巴母細胞系，可用于白血病發(fā)病機制的研究。;傳代方法：１：２傳代;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：X63細胞、EFO 27細胞、LL/2(LLc1)細胞

C41 Cells;背景說明：宮頸鱗癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HEK293-A細胞、C2A細胞、EMT6細胞

NCIH1648 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３-１：６傳代;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：NRK-49F細胞、Hs343T細胞、NS-1細胞

SUM102 Cells;背景說明：乳腺癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：CPAE細胞、GS9L細胞、H2.35細胞

Metastatic Variant-522 Cells;背景說明：肺腺癌；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCI-H2291細胞、GH 3細胞、Granta 519細胞

GM20000 Cells(提供STR鑒定圖譜)

HAP1 IRF2BPL (-) 1 Cells(提供STR鑒定圖譜)

SKUT-1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：４-１：１２傳代，２天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：H-498細胞、Kit225細胞、Ra #1細胞

SW-626 Cells;背景說明：卵巢癌;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Malme-3 M細胞、TALL 1細胞、SK-N-BE(2)細胞

NCI-H1954 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：TE13細胞、HUT-226細胞、PCI:SG-231細胞

H-209 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：４傳代；每周換液２次。;生長特性：懸浮生長，有少數(shù)細胞疏松貼壁;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：253J-BV細胞、UCLA RO-81A-1細胞、KS-1 [Human glioblastoma]細胞

CHP-100 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：PC9細胞、CAL-33細胞、HPDE6c7細胞

Hepa1-6 Cells;背景說明：此細胞株源自C５７/L小鼠中引發(fā)的BW７７５６肝癌；表達AFP、α１抗胰蛋白酶、淀粉酶；鼠痘病毒陰性。此細胞可以在無血清的培養(yǎng)基中繁殖，培養(yǎng)基成分是：DMEM,７５%;Waymouth＇sMAB８７/３培養(yǎng)基，２５%。添加３x１０-８M。;傳代方法：１：２傳代;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：Dx5細胞、CATH-a細胞、SAS細胞

G361mel Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：４傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：VP 267細胞、HCC-202細胞、HPB/ALL細胞

NKT Cells;背景說明：NK Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：MASMC細胞、Tca8113細胞、SKMEL5細胞

HPS0947 Cells(提供STR鑒定圖譜)

JoN HDF Cells(提供STR鑒定圖譜)

MDCC-HP8 Cells(提供STR鑒定圖譜)

ND41657 Cells(提供STR鑒定圖譜)

PSFDFN714 Cells(提供STR鑒定圖譜)

UCSD041i-33-2 Cells(提供STR鑒定圖譜)

ZR-75-1 VIII-24 Cells(提供STR鑒定圖譜)

HCM-SqCC010 Cells(提供STR鑒定圖譜)

RPMI 1788 Cells;背景說明：B淋巴細胞；EBV轉(zhuǎn)化;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Ca Ski細胞、BIU-87/Adr細胞、GM637A細胞

NTERA-2/D1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RM-1細胞、H-520細胞、MAVER-1細胞

Hs1.Tes Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代;生長特性：貼壁生長　;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：hCMEC/D3細胞、UT7細胞、RPTEC/TERT 1細胞

CW-2 Cells;背景說明：來源于結(jié)腸癌。 CEA陽性，移植于裸鼠可成瘤。;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：NCIH2066細胞、C-4 I細胞、Rat Chondrosarcoma Swarm細胞

H-748 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：３-４天換液１次。;生長特性：懸浮生長 ;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Mel624細胞、MARC 145細胞、SK-RC 39細胞

H-748 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：３-４天換液１次。;生長特性：懸浮生長 ;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Mel624細胞、MARC 145細胞、SK-RC 39細胞

B16 melanoma F10 Cells;背景說明：B１６-F１０是B１６-F０的亞系。;傳代方法：消化３-５分鐘。１：２。３天內(nèi)可長滿。;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NCIH69細胞、OKT3細胞、EFO-27細胞

2PK3 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NIH 3T6細胞、Lewis-Lung細胞、NTERA2D1細胞

Murine Thymic Epithelial Cell line 1 Cells;背景說明：胸腺；上皮細胞；自發(fā)永生;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：T-84細胞、Lilly Laboratories Cell-Porcine Kidney 1細胞、PA I細胞

SU.86.86 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２傳代；;生長特性：貼壁生長;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：D6P2T細胞、U031細胞、IMR90細胞

H661 Cells;背景說明：該細胞１９８２年建系，源自一位患有大細胞肺癌的男性的胸腔積液。該細胞角蛋白、波形蛋白陽性。;傳代方法：１：３—１：５傳代，每周換液２—３次;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：ABC1細胞、BHK21細胞、TSUpr1細胞

H-740 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：UACC 812細胞、H-1436細胞、HNE-2細胞

LIXC-002 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：３傳代,３-４天傳１次;生長特性：貼壁生長;形態(tài)特性：上皮細胞樣;相關(guān)產(chǎn)品有：B4G12細胞、MRC9細胞、M059J細胞

Hs 822.T Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：４傳代；每周換液２-３次。;生長特性：貼壁生長;形態(tài)特性：上皮樣;相關(guān)產(chǎn)品有：SK-N-MC細胞、CV 1細胞、GCT0404細胞

PC 61 5.3 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NE1-E6E7細胞、ST2細胞、HT 1197細胞

SMUSHi005-A Cells(提供STR鑒定圖譜)

MODE-K Cells;背景說明：小腸；上皮 Cells;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RC-4B細胞、GM03190A細胞、H-69細胞

GA-10(Clone 4) Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：每周換液兩次;生長特性：懸浮生長 ;形態(tài)特性：淋巴母細胞樣;相關(guān)產(chǎn)品有：hTERTHME1細胞、UCH1細胞、MKN-45細胞

HEL-92-1-7 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：每周２-３次。;生長特性：懸浮生長;形態(tài)特性：成淋巴細胞;相關(guān)產(chǎn)品有：HCC-70細胞、Panc-3_27細胞、BNL.1ME A.7R.1細胞

NCTC 929 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Panc02.03細胞、HPMEC細胞、S3 HeLa細胞

Hs 695.T Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：４傳代，２-３天換液１次。;生長特性：貼壁生長;形態(tài)特性：上皮細胞;相關(guān)產(chǎn)品有：HSC6細胞、IBRS-2細胞、CHL-11細胞

SupT1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：２-３天換液１次。;生長特性：懸浮生長;形態(tài)特性：淋巴母細胞樣　;相關(guān)產(chǎn)品有：HB611細胞、HCC1171細胞、H4-IIE-C3細胞

EFM-192A Cells;背景說明：乳腺癌；胸腔積液轉(zhuǎn)移；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：H524細胞、Anip 973細胞、Mv1Lu細胞

MF2059 Cells;背景說明：皮膚；T淋巴細胞瘤;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：懸浮;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：WM266mel細胞、HBL 100細胞、SU.86細胞

MDA-MB-231人乳腺癌細胞全年復蘇|已有STR圖譜

OVTOKO Cells;背景說明：卵巢透明細胞癌；脾轉(zhuǎn)移；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：RL-65細胞、Tn 5B1-4細胞、ketr 3細胞

TE 85 ClF-5 Cells;背景說明：骨肉瘤；女性;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：IGROV細胞、UMNSAH-DF 1細胞、DMS 79細胞

IGR.OV1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：HT 1197細胞、ST2細胞、SEG-1細胞

G 292 Clone A 141B1 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：T-HEECs細胞、CESS細胞、SUP-T1細胞

H19-7 Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：Lilly Laboratories Cell-Monkey Kidney 2細胞、JM1細胞、BNL-HCC細胞

LCLC-103H Cells;背景說明：詳見相關(guān)文獻介紹;傳代方法：１：２-１：３傳代；每周換液２-３次。;生長特性：貼壁或懸浮，詳見產(chǎn)品說明書部分;形態(tài)特性：詳見產(chǎn)品說明書;相關(guān)產(chǎn)品有：NIH 3T3細胞、NCIH747細胞、MDA-MB435細胞

BayGenomics ES cell line CSI001 Cells(提供STR鑒定圖譜)

BayGenomics ES cell line RST202 Cells(提供STR鑒定圖譜)

BTLA6.4 Cells(提供STR鑒定圖譜)

L2-10C1 Cells(提供STR鑒定圖譜)

Pro104.K81.15 Cells(提供STR鑒定圖譜)

Rat-F Cells(提供STR鑒定圖譜)

" "PubMed=1000506

Lippman M.E., Bolan G., Huff K.

The effects of androgens and antiandrogens on hormone-responsive human breast cancer in long-term tissue culture.

Cancer Res. 36:4610-4618(1976)

PubMed=833871; DOI=10.1093/jnci/58.2.209

Fogh J., Wright W.C., Loveless J.D.

Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x

Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.

Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

Tissue Antigens 11:279-286(1978)

PubMed=730202; DOI=10.1007/BF02616120

Cailleau R.M., Olive M., Cruciger Q.V.J.

Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization.

In Vitro 14:911-915(1978)

PubMed=7000337

Brinkley B.R., Beall P.T., Wible L.J., Mace M.L. Jr., Turner D.S., Cailleau R.M.

Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro.

Cancer Res. 40:3118-3129(1980)

PubMed=6935474; DOI=10.1093/jnci/66.2.239

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