Human Kremen-1 protein, Fc Tag

分子別名（Synonym）

KREMEN1,KRM1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Kremen-1, Fc Tag (KR1-H5253) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Thr 392 (Accession # Q96MU8-1).

Predicted N-terminus: Arg 21

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 67.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Kremens是Dickkopf 1（Dkk1）的高親和力受體，通過下調(diào)低密度脂蛋白受體相關(guān)蛋白6（LRP6）來調(diào)節(jié)Wnt/β-catenin信號(hào)通路。Kremen1被認(rèn)為是脊椎動(dòng)物通過LRP6調(diào)節(jié)經(jīng)典Wnt信號(hào)傳導(dǎo)的膜復(fù)合物的一個(gè)組成部分。此外，KREMEN1（KRM1）也被確定為柯薩奇病毒A10（CV-A10）的功能性受體，柯薩奇病毒是手足口?。?/span>HFMD）的病原體。