Human Fetuin B Protein, His Tag

分子別名（Synonym）

FETUB,Fetuin-B,16G2,Gugu

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human Fetuin B, His Tag (FEB-H52H7) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 382 (Accession # AAH69670 ).

Predicted N-terminus: Cys 16

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胎球蛋白B（FETUB）是哺乳動物胎球蛋白家族的第二個成員，也被稱為胎球蛋白樣蛋白IRL685、16G2和Gugu，在肝臟和睪丸中表達(dá)，含有2個半胱氨酸蛋白酶抑制劑胎球蛋白-B型結(jié)構(gòu)域。FETUB是卵子受精所需的蛋白酶抑制劑。FETUB還需要在受精前防止透明帶過早硬化，可能是通過抑制ASTL的蛋白酶活性，ASTL是一種介導(dǎo)ZP2切割并引發(fā)透明帶硬化的蛋白酶（通過相似性）。作為一種肝臟來源的血漿蛋白，FETUB對受精至關(guān)重要。