Human TRGC1 Protein, His Tag

分子別名（Synonym）

TRGC1

表達區(qū)間及表達系統(tǒng)（Source）

Human TRGC1, His Tag (TR1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 1- Ala 138 (Accession # P0CF51-1).

Predicted N-terminus: Asp 1

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 17.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

跨膜蛋白TCR由兩條二硫鍵連接的多肽鏈組成：α和β鏈，γ和δ鏈。每條多肽鏈由一個可變區(qū)和一個恒定區(qū)組成。TRGC1是T細胞受體（TCR）γ鏈的恒定區(qū)。它識別上皮邊界經(jīng)常表達的非肽抗原，這意味著激活γδT細胞的抗原大多是MHC非依賴性的。γδT細胞在人類和小鼠中具有廣泛的功能，包括皮膚和粘膜上皮傷口修復(fù)、耐受誘導(dǎo)、細胞毒性和調(diào)節(jié)免疫反應(yīng)的各種細胞因子的產(chǎn)生。