Mouse Integrin alpha 2 beta 1 (ITGA2&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子別名（Synonym）
Integrin alpha 2 beta 1,ITGA2 & ITGB1
表達區(qū)間及表達系統(tǒng)（Source）
Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-M52W3) is expressed from human 293 cells (HEK293). It contains AA Tyr 27 - Thr 1129 (ITGA2) & Gln 21 - Asp 728 (ITGB1) (Accession # Q62469-1 (ITGA2) & P09055-1 (ITGB1)).
Predicted N-terminus: Tyr 27 (ITGA2) & Gln 21 (ITGB1)
蛋白結構（Molecular Characterization）
Mouse ITGA2&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA2 and ITGB1, has a calculated MW of 127.6 kDa (ITGA2) and 83.5 kDa (ITGB1). Subunit ITGA2 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 140-150 kDa (ITGA2) and 100-120 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
內毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
整合素α2β1是共享β1亞基的十二個整合素家族粘附受體之一。它是層粘連蛋白、膠原蛋白、膠原蛋白C-前肽、纖維連接蛋白和E-鈣粘蛋白的受體。它識別膠原蛋白中的脯氨酸羥基化序列G-F-P-G-E-R。整合素ITGA2:ITGB1是人輪狀病毒a和人回聲病毒1和8的受體。DGEA抑制輪狀病毒與α2β1的結合和感染性。在一個新的過程中，使用整合素的病毒通過VP4中的DGE結合α2β1的α2 I結構域，并通過VP7與αXβ2（通過GPR）和αVβ3相互作用，以促進細胞進入和感染。
關鍵字： Integrin a2b1;a2b1蛋白;整合素a2b1;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產業(yè)領域提供關鍵生物試劑產品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產基地。目前累計服務客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。