Mouse LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)
分子別名（Synonym）
LRRC32 & TGF-beta 1,LRRC32&TGFB1
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Mouse LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (GA1-M52W2) is expressed from human 293 cells (HEK293). It contains AA Ile 18 - Asn 628 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1) (Accession # G3XA59-1 (LRRC32) & P04202-1 (TGF-beta 1)).
Predicted N-terminus: Leu 30
蛋白結(jié)構(gòu)（Molecular Characterization）
Mouse LRRC32&TGFB1 Heterotrimer Protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.7 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 80 kDa (LRRC32), 40 kDa (LAP) and 14 kDa (mature TGF-beta 1) due to glycosylation respectively.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
GARP（LRRC32）是一種跨膜蛋白，結(jié)合潛在的TGF-β1并將其固定在Treg表面。并已被證明可以促進(jìn)轉(zhuǎn)化生長因子β（TGF-β）的活化和分泌。GARP在表面活化的Tregs上高度表達(dá)，并增強(qiáng)Tregs的抑制功能。此外，GARP可以與潛在的轉(zhuǎn)化生長因子β（TGF-β）結(jié)合，從而促進(jìn)TGF-β的分泌和激活。TGF-β對Tregs的穩(wěn)態(tài)和功能起著關(guān)鍵作用。值得注意的是，還觀察到表達(dá)GARP/潛在TGF-β1復(fù)合物的成纖維細(xì)胞和內(nèi)皮細(xì)胞系不會激活TGF-β1。然而，不能排除需要特定的刺激來觸發(fā)這些細(xì)胞類型表面復(fù)合物的TGF-β1激活。
關(guān)鍵字： LRRC32&TGF-beta 1;LRRC32蛋白;TGF-beta 1蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。