Mouse IL-2 Protein, His Tag

分子別名（Synonym）

IL2,TCGF,lymphokine,Interleukin 2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-2, His Tag (IL2-M52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Gln 169 (Accession # P04351-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.1 kDa. The protein migrates as 22-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-2（IL-2）是一種白介素，一種細(xì)胞因子免疫系統(tǒng)信號(hào)分子，是一種促白細(xì)胞激素，在身體對(duì)微生物感染的自然反應(yīng)中起著重要作用，并有助于區(qū)分外來(lái)（非自身）和自身。IL-2通過(guò)與淋巴細(xì)胞（負(fù)責(zé)免疫的細(xì)胞）表達(dá)的IL-2受體結(jié)合來(lái)介導(dǎo)其作用。成熟的人IL-2與小鼠和大鼠IL-2的aa序列同源性分別為56%和66%。人和小鼠IL-2具有跨物種活性。IL-2受體由三個(gè)亞基組成，它們以不同的預(yù)形成復(fù)合物存在于細(xì)胞表面。IL-2在胸腺T細(xì)胞發(fā)育過(guò)程中也是必要的，用于成熟被稱為調(diào)節(jié)性T細(xì)胞（T-reg）的獨(dú)特T細(xì)胞亞群。從胸腺中排出后，T-Regs的功能是防止其他T細(xì)胞識(shí)別和對(duì)抗“自身抗原”，這可能會(huì)導(dǎo)致“自身免疫”。T-Regs通過(guò)阻止反應(yīng)細(xì)胞產(chǎn)生IL-2來(lái)實(shí)現(xiàn)這一目的。因此，需要IL-2來(lái)區(qū)分自我和非自我，這是免疫系統(tǒng)的另一個(gè)獨(dú)特特征。