Mouse IL-13 Protein, His Tag

分子別名（Synonym）

IL13,ALRH,BHR1,MGC116786,MGC116788,MGC116789,P600,Interleukin-13

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse IL-13, His Tag (IL3-M5249) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Phe 131 (Accession # P20109-1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.2 kDa. The protein migrates as 16-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素13（IL13）也稱為ALRH、BHR1和P600，是一種單鏈糖基化多肽，是調(diào)節(jié)炎癥和免疫反應(yīng)的關(guān)鍵細(xì)胞因子。IL13由許多細(xì)胞類型分泌，但尤其是由T輔助型2（Th2）細(xì)胞分泌。IL-13通過多亞基受體誘導(dǎo)其作用，該受體包括IL-4受體的α鏈（IL-4Rα）和兩個已知的IL-13特異性結(jié)合鏈中的至少一個。IL-13的功能與IL-4的功能有很大重疊，特別是在造血細(xì)胞上誘導(dǎo)的變化方面，但鑒于IL-4的作用更為強(qiáng)大，這些作用可能不那么重要。IL-13誘導(dǎo)基質(zhì)金屬蛋白酶（MMPs），作為防止易導(dǎo)致窒息的過度過敏性炎癥機(jī)制的一部分。IL-13誘導(dǎo)過敏性肺病的許多特征，包括氣道高反應(yīng)性、杯狀細(xì)胞化生和粘液高分泌，這些都會導(dǎo)致氣道阻塞。