HRV-3C Protease, GST Tag (1U/μL) (active enzyme)
產品描述（Product Details）
HRV 3C Protease is highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue.
應用說明（Application）
Removal of purification tags from fusion proteins
單位定義（Unit Definition）
One unit will cleave >95% of 100 μg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4℃ for 16 h.
純度（Purity）
>95% as determined by SDS-PAGE.
酶活（Enzyme Activity）
>1 U/μL
內毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
存儲（Storage）
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state.
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
Protease Recombinant is fusion protein of GST and human rhinovirus (HRV) type 14 3C protease.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein. The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.
關鍵字： 3C (HRV);3C (HRV)蛋白酶;3C (HRV)蛋白;ACRO;百普賽斯;
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