熒光
λex?337?nm; λem?492?nm in chloroform (after derivatization with hexylamine)
一般描述
Dansyl Chloride, also known as 5-dimethylamino naphthalene-1-sulfonyl chloride, is a pre-column derivatization reagent. After the derivatization reaction, the fluorescent derivatives are analyzed using several analytical methods like NMR, IR, UV-vis, and fluorescence microscopy. Dansyl Chloride is a typical aromatic sulphonyl chloride that reacts with various bases, resulting in derivatives with variable stability.
應(yīng)用
丹磺酰氯(DNSC)是一種伯胺反應(yīng)劑,廣泛應(yīng)用于氨基酸和肽的N端衍生,以及利用反相HPLC等分析方法進(jìn)行的多胺檢測(cè)。 一種用于氨基酸和肽的熒光N-末端衍生化的熒光試劑,并且可用反相HPLC進(jìn)行檢測(cè)。
Sigma-Aldrich西格瑪奧德里奇(上海)貿(mào)易有限公司
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