D-Luciferin potassium NEW
| Price | $41 | $74 | $105 |
| Package | 10mg | 50mg | 100mg |
| Min. Order: | |
| Supply Ability: | 10g |
| Update Time: | 2024-11-19 |
Product Details
| Product Name: D-Luciferin potassium | CAS No.: 115144-35-9 |
| Purity: 99.93% | Supply Ability: 10g |
| Release date: 2024/11/19 |
Product Introduction
Bioactivity
| Name | D-Luciferin potassium |
| Description | D-Luciferin potassium (D-Luciferin K Salt) is a popular bioluminescent substrate of luciferase in the presence of ATP. |
| Kinase Assay | Enolase activity assay is in the 2.0 mM MgSO4 and 400 mM KCl in the absence or presence of ENOblock or NaF, at 37°C by incubating pure enolase (3–9 U) in a buffer containing 50 mM imidazole-HCl (pH 6.8). The reaction is initiated by adding 1 μmol of 2-phospho-D-glycerate, and the OD is measured after 10 min of reaction time with a spectrophometer at 240 nm. |
| In vitro | METHODS: Preparation of D-Luciferin potassium for in vitro bioluminescence analysis: 1. Prepare 200× D-Luciferin reserve solution (30 mg/mL) with sterile water and mix gently until all dissolved. It can be used immediately after preparation or stored at -20℃. 2. Prepare 1× D-Luciferin (150 μg/mL) working solution in preheated tissue culture medium. 3. Aspirate the medium from the cultured cells. 4. Add 1× D-Luciferin solution to the cells before imaging. Incubate the cells at 37°C for a short period of time before imaging to increase signal. |
| In vivo | METHODS: Preparation of D-Luciferin potassium for in vivo bioluminescence analysis: 1. Prepare a fresh 15 mg/mL D-Luciferin stock solution in DPBS. Sterilize by filtering with 0.20 um filter. 2. Inject 10 μL/g of mouse body weight. 150 mg D-Luciferin/kg body weight per mouse. For example, for a mouse weighing 10 g, inject 100 μL to provide 1.5 mg D-Luciferin. 3. inject D-Luciferin intraperitoneally 10-15 min prior to imaging. fluorescein kinetic studies should be performed for eah animal model to determine peak signal times. |
| Storage | keep away from direct sunlight,store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 2.63 mg/mL (8.26 mM), Sonication is recommended. H2O : 50 mg/mL (157 mM), Sonication is recommended. |
| Keywords | inhibit | DLuciferin potassium | Firefly luciferin | D-Luciferin | D-Luciferin potassium | D Luciferin potassium | Beetle Luciferin | Inhibitor |
Company Profile Introduction
Target Molecule Corp. (TargetMol) is a global high-tech enterprise, headquartered in Boston, MA, specializing in chemical and biological research product and service to meet the research needs of global customers.
TargetMol has evolved into one of the biggest global compound library and small molecule suppliers and a customer based on 40+ countries. TargetMol offers over 80 types of compound libraries and a wide range of high-quality research chemicals including inhibitors, activator, natural compounds, peptides, inhibitory antibodies, and novel life-science kits, for laboratory and scientific use. Besides, virtual screening service is also available for customers who would like to conduct the computer-aided drug discovery.
You may like
Recommended supplier
| Product name | Price | Suppliers | Update time | |
|---|---|---|---|---|
| $0.00/1KG |
VIP3Y
|
Hebei Weibang Biotechnology Co., Ltd
|
2024-10-24 | |
| $0.00/25KG |
VIP5Y
|
Hebei Mujin Biotechnology Co.,Ltd
|
2023-11-14 | |
| $30.00/1kg |
Anhui Ruihan Technology Co., Ltd
|
2023-09-08 | ||
| $0.00/10G |
VIP4Y
|
WUHAN FORTUNA CHEMICAL CO., LTD
|
2023-02-15 | |
| $1.00/1kg |
VIP7Y
|
Career Henan Chemical Co
|
2018-12-23 | |
| $1.00/1kg |
VIP7Y
|
Career Henan Chemical Co
|
2018-12-21 |
United States- Since: 2011-01-07
- Address: 36?Washington?Street, Wellesley?Hills
INQUIRY