1179347-65-9
1179347-65-9 結(jié)構(gòu)式
基本信息
5-{2',6'-dihydroxy-[1,1'-biphenyl]-4-yl}-1H-indole-3-carbonitrile
1H-Indole-3-carbonitrile, 5-(2',6'-dihydroxy[1,1'-biphenyl]-4-yl)-
常見(jiàn)問(wèn)題列表
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AMPK 25 μM (EC 50 ) |
mTORC1
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MT 63-78 (0-50 μM; 4 days; LNCaP and PC3 cells) treatment shows a dose-dependent decrease in cell number, and concomitant to the activation of AMPK signaling.
MT 63-78 (25 μM; 24 hours; LNCaP and CRPC cells) treatment induces a significant enrichement in the G2/M population.
MT 63-78 (0-50 μM; 24 hours; LNCaP, PC3, C4-4, C4-2B, CL1and 22RV1cells) treatment induces reduction of anti-apoptotic Mcl-1 in concert with accumulation of the pro-apoptotic BH3-only protein Puma.
MT 63-78 (0-50 μM; 30 minutes; LNCaP and PC3 cells) treatment shows a dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792. And also increases Thr172 phosphorylation on the AMPK α subunit.
Cell Viability Assay
| Cell Line: | LNCaP and PC3 cells |
| Concentration: | 0 μM, 1 μM, 5 μM, 10 μM, 25 μM, 50 μM |
| Incubation Time: | 4 days |
| Result: | A dose-dependent decrease in cell number, concomitant to the activation of AMPK signaling was observed. |
Cell Cycle Analysis
| Cell Line: | LNCaP and CRPC cells |
| Concentration: | 25 μM |
| Incubation Time: | 24 hours |
| Result: | Induced a significant enrichement in the G2/M population in both androgen sensitive and CRPC cell models. |
Apoptosis Analysis
| Cell Line: | LNCaP, PC3, C4-4, C4-2B, CL1and 22RV1cells |
| Concentration: | 0 μM, 10 μM, 25 μM, 50 μM |
| Incubation Time: | 24 hours |
| Result: | Induced reduction of anti-apoptotic Mcl-1 in concert with accumulation of the pro-apoptotic BH3-only protein Puma in all PCa cells. |
Western Blot Analysis
| Cell Line: | LNCaP and PC3 cells |
| Concentration: | 0 μM, 0.25 μM, 0.5 μM, 1 μM, 5 μM, 25 μM, 50 μM |
| Incubation Time: | 30 minutes |
| Result: | Observed a dose-dependent phosphorylation of the two major AMPK targets Acetyl-CoA Carboxylase (ACC) on Ser79 and of Raptor on Ser792. A corresponding increase in Thr172 phosphorylation on the AMPK α subunit was also observed. |
MT 63-78 (30 mg/kg; intraperitoneal injection; daily; for 14 days; C57 BL/6 male mice) treatment leads to a 33% inhibition of tumor growth.
| Animal Model: | C57 BL/6 male mice bearing LNCaP tumors |
| Dosage: | 30 mg/kg |
| Administration: | Intraperitoneal injection; daily; for 14 days |
| Result: | Led to a 33% inhibition of tumor growth. |