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115532-49-5

中文名稱 TMRM
CAS 115532-49-5
分子式 C25H25N2O3
115532-49-5 結(jié)構(gòu)式 115532-49-5 結(jié)構(gòu)式

常見問題列表

生物活性
TMRM 是一種細(xì)胞滲透性陽離子親脂性紅色熒光染料 (λex=530 nm, λem=592 nm)。
體外研究

TMRM is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of safranin or TMRM shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%. Exposure of hippocampal cultures to low concentrations of TMRM (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM (1 to 25 μM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 μM) enhance apoptosis (K D =?500 nM).

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