| Identification | Back Directory | [Name]
Deoxyribonuclease | [CAS]
9003-98-9 | [Synonyms]
dnasw
DNASE
dnaase
DNaseA
Dnase 1
DNAaseI
dornase
dornava
DNASE I
dornavac
RNase free
EC 3.1.21.1
EC: 3.1.4.5
dnanuclease
IUB: 3.1.21.1
CHRYSENE NEAT
DNASE I DN-25
DNASE I DN-EP
alkalinednase
DNASE I TYPE II
DNASE I TYPE IV
dnadepolymerase
dnaendonuclease
DNASE I, BOVINE
Bovine pancrease
pancreatic Dnase
deoxyribo-nucleas
DNASE I TYPE II-S
pancreaticdornase
DEOXYRIBONUCLEASE
DeoxyribonueleaseⅠ
deoxyribonucleasea
DESOXYRIBONUCLEASE
RNASE FREE DNASE I
froM Bovine Spleen
DEOXYRIBONUCLEASE 1
DEOXYRIBONUCLEASE I
Nuclease, deoxyribo-
DNASE I (RNASE-FREE)
DNASE I, DS QUALIFIED
Deoxyribonuclease
endodeoxyribonucleasei
DNASE I BOVINE PANCREAS
DEOXYRIBONUCLEASE I DN-25
DEOXYRIBONUCLEASE I DN-EP
alkalinedeoxyribonuclease
Deoxyribonuclease (DNase)
Deoxyribonuclease Pancreas
Deoxyribonuclease USP/EP/BP
DNase I(DeoxyribonueleaseⅠ)
deoxyribonucleicphosphatase
DEOXYRIBONUCLEASE I TYPE II
DEOXYRIBONUCLEASE I TYPE IV
DEOXYRIBONUCLEODEPOLYMERASE
DEOXYRIBONUCLEASE I, BOVINE
escherichiacoliendonucleasei
DeoxyribonueleaseⅠ (Dnase I)
PANCREATIC DEOXYRIBONUCLEASE
DNase I, froM Bovine Pancreas
deoxyribonuclease(pancreatic)
DNase I (Deoxyribonuclease I)
5'-OLIGO-NUCLEOTIDE HYDROLASE
DEOXYRIBONUCLEASE I TYPE II-S
Deoxyribonucleasebovinepancreas
DEOXYRIBONUCLEASE I FROM BOVINE
DEOXYRIBONUCLEASE, BOVINE SPLEEN
Deoxyribonucleasefrombovinespleen
BENZO(A)PYRENE 50UG/ML IN TOLUENE
Native Bovine Deoxyribonuclease I
DeoxyribonucleaseIExBovinePancreas
deoxyribonucleaseif.bovinepancreas
Deoxyribonucleasefrombovinepancreas
DEOXYRIBONUCLEASE I, BOVINE PANCREAS
High active Deoxyribonuclease / DNase
deoxyribonuclease I, bovine pancrease
Recombinant Human Deoxyribonuclease I
DNAase, ≥2,000 Kunitz units/mg protein
DNase-S recombinant, min. 500.000 U/mg
DEOXYRIBONUCLEASE I, BOVINE, RECOMBINANT
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE
Deoxyribonucleate 6′-oligonucleotido-hydrolase
DEOXYRIBONUCLEASE I TYPE IV FROM*BOVINE PANCREAS
DEOXYRIBONUCLEASE I TYPE II FROMBOVINE P ANCREAS
deoxyribonuclease I type ii-S from*bovine pancrea
DEOXYRIBONUCLEASE I FROM BOVINE PANCREAS , 10000 U
Deoxyribonuclease I from Bovine Pancreas(Filtered)
DEOXYRIBONUCLEASE I FROM BOVINEPANCREAS STANDARDIZ
Deoxyribonuclease I from bovine pancreas, lyophil.
DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, ~1800 U/MG*
DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, ~2000 U/MG*
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE DN-25
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE DN-EP
DEOXYRIBONUCLEASE I FROM BOVINE PANCREASE, TYPE II-S
DEOXYRIBONUCLEASE I FROM BOVINE PANCREAS , ~2500 U/MG
Deoxyribonuclease I from bovine pancreas(Recombinant)
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE TYPE II
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE TYPE IV
Native Bovine Deoxyribonuclease I RNase-free solution
Deoxyribonuclease I from Bovine pastoris, Recombinant
DEOXYRIBONUCLEASE I ACTIVITY: 10000+ UNIT/G PH:5.5~6.0
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDO-HYDROLASE, BOVINE
DEOXYRIBONUCLEATE 5'-OLIGONUCLEOTIDOHYDROLASE TYPE II-S
Deoxyribonuclease I, from bovine pancreas, >=2000 KU/mg
DEOXYRIBONUCLEASE I F. BOVINE PANCREAS, LYOPH. ~3000 U/MG
Deoxyribonucleate 5μ-oligonucleotido-hydrolase, DNase I
deoxyribonuclease i rnase-free solution from bovine pancreas
Deoxyribonuclease I from Bovine Pancreas(Recombinant,Solution)
Deoxyribonuclease I from Bovine Pancreas(Ribonuclease&Protease Free)
Deoxyribonuclease I froM Bovine Pancreas(RNase & Protease Free,Solution)
deoxyribonuclease
Deoxyribonucleate 5′-oligonucleotido-hydrolase
DNase I
Deoxyribonuclease I from bovine pancreas,DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
Deoxyribonuclease I froM Bovine Pancreas Min. 3000 Kunits units/Mg PANCREAS 3000 KUNITZ UNITS/MG LYOPHIL. | [EINECS(EC#)]
232-667-0 | [Molecular Formula]
NULL | [MDL Number]
MFCD00130918 |
| Chemical Properties | Back Directory | [density ]
100 g/mL at 20 °C
| [vapor pressure ]
0.004Pa at 25℃ | [storage temp. ]
−20°C
| [solubility ]
Soluble in 0.15 M sodium chloride (5.0mg/L) clear. | [form ]
solution (clear, colorless)
| [color ]
white
| [Water Solubility ]
125g/L at 25℃ | [Merck ]
13,2923 | [EPA Substance Registry System]
Nuclease, deoxyribo-(9003-98-9) |
| Hazard Information | Back Directory | [Uses]
Deoxyribonuclease I is used for the removal of DNA from protein samples. DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis | [General Description]
DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.
- Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
- Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
- DNase I structure resembles the structure of to exonuclease III. It includes two central ? sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]
| [Flammability and Explosibility]
Notclassified | [Biochem/physiol Actions]
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. | [Clinical Use]
DNAse is a human endonuclease, normally present insaliva, urine, pancreatic secretions, and blood. The enzymecatalyzes the hydrolysis of extracellular DNA into oligonucleotides.Aerosolized recombinant human deoxyribonucleaseI (rhDNAse), dornase alfa, Pulmozyme, has beenformulated into an inhalation agent for the treatment of pulmonarydisease in patients with CF.Among the clinical manifestations of CF are obstructionof the airways by viscous, dehydrated mucus. Pulmonaryfunction is diminished, and microbes can become entrappedin the viscid matrix. A cycle of pulmonary obstruction andinfection leads to progressive lung destruction and eventualdeath before the age of 30 for most CF patients. The immunesystem responds by sending in neutrophils, and theseaccumulate and eventually degenerate, releasing largeamounts of DNA. The high levels of extracellular DNA releasedand the mucous glycoproteins are responsible for thedegenerating lung function. The DNA-rich secretions alsobind to aminoglycoside antibiotics typically used to treat theinfections. In vitro studies showed that the viscosity of thesecretions could be reduced by application of DNAse I.
Pulmozyme is approved for use in the treatment of CFpatients, in conjunction with standard therapies, to reducethe frequency of respiratory infections requiring parenteralantibiotics and to improve pulmonary function. The dose isdelivered at a level of 2.5 mg daily with a nebulizer.Pulmozyme is not a replacement for antibiotics, bronchodilators,and daily physical therapy. |
|
|