| Identification | Back Directory | [Name]
P-NITROPHENYL BUTYRATE | [CAS]
2635-84-9 | [Synonyms]
4-NITROPHENYL BUTYRATE
P-NITROPHENYL BUTYRATE
4-Nitrophenol butyrate
PARA-NITROPHENYLBUTYRATE
(4-nitrophenyl) butanoate
4-Nitrophenyl butyrate >=98%
BUTYRIC ACID 4-NITROPHENYL ESTER
Butanoic acid p-nitrophenyl ester
Butanoic acid, 4-nitrophenyl ester
p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester
4-Nitrophenyl butyrate,p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester | [EINECS(EC#)]
258-258-3 | [Molecular Formula]
C10H11NO4 | [MDL Number]
MFCD00047734 | [MOL File]
2635-84-9.mol | [Molecular Weight]
209.2 |
| Chemical Properties | Back Directory | [Boiling point ]
184-185 °C | [density ]
1.19 g/mL at 20 °C(lit.)
| [storage temp. ]
-20°C | [solubility ]
chloroform: 0.1 g/mL, clear, faintly yellow
| [form ]
A neat oil | [color ]
Colorless to light yellow | [BRN ]
1879729 | [InChIKey]
DVDUMIQZEUTAGK-UHFFFAOYSA-N |
| Hazard Information | Back Directory | [Description]
P-nitrophenyl butyrate also kown as 4-nitrophenyl butyrate, is a probe for lipase activity. It is hydrolyzed by lipase in the water ambient condition, giving rise to p-nitrophenol and butyric acid. P-nitrophenol actually occurs in the anionic form, p-nitrophenolate, giving a yellowish coloration and absorbing light at 410 nm in an alkaline buffer such as Tris-HCl pH 7.5. | [Uses]
4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm. | [Uses]
A probe for lipase activity. | [Definition]
ChEBI: A butyrate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of butyric acid. | [General Description]
4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm. | [References]
[1] POHANKA M. Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review.[J]. Molecules, 2019. DOI:10.3390/molecules24030616.
[2] WENTING SHANG. Enzymatic hydrolysis of p-nitrophenyl butyrate in CO2-based micelle systems[J]. Journal of chemical technology and biotechnology, 2013, 89 9: 1324-1329. DOI:10.1002/jctb.4246.
[3] SIGRUN H. STERRI Frode F Bj?rn A Johnsen. A radiochemical assay method for carboxylesterase, and comparison of enzyme activity towards the substrates methyl [1-14C] butyrate and 4-nitrophenyl butyrate[J]. Biochemical pharmacology, 1985, 34 15: Pages 2779-2785. DOI:10.1016/0006-2952(85)90579-9.
[4] BISHNU PRASAD PANDEY. Bergenia pacumbis from Nepal, an astonishing enzymes inhibitor.[J]. BMC Complementary Medicine and Therapies, 2020: 198. DOI:10.1186/s12906-020-02989-2. |
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